A3803

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, lyophilized powder, essentially fatty acid free, ≥98% (agarose gel electrophoresis)

Synonym(s):
Albumin bovine serum, Bovine albumin, BSA
CAS Number:
EC Number:
MDL number:

Quality Level

biological source

bovine

assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

application(s)

cell culture | mammalian: suitable

impurities

≤0.005% Fatty acids

loss

≤5.0%, Loss on drying

pH

7

solubility

water: soluble (40 mg/ml)

suitability

suitable for ELISA applications (as diluent)

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Bovine serum albumin (BSA) is a 66.4 kDa, water-soluble protein composed of 583 amino acids. It has a single polypeptide chain with three homologous domains formed by six α-helices. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating. BSA has been used as a model protein in various applications, including immunodiagnostic procedures, cell culture media and clinical chemistry. Serum albumins facilitates drug disposition and efficacy by maintaining osmotic blood pressure.

Application

Bovine Serum Albumin has been used:
  • to prevent nonspecific adsorption of fibrin matrices to tissue culture plates
  • as a constituent in the preparation of albumin droplets
  • as a supplement in L-15 medium to increase spermatogonia number without enhancing the growth of somatic cells
  • as a standard protein to evaluate metal oxide adsorption isotherms

Among its many applications, BSA is a fatty acid (FA) carrier. Thus when BSA is used in applications like cell culture, it is important to exercise control over particular FA′s for cell culture, because specific fatty acids can affect different cell lines in a variable manner. In order to control fatty acid content in cell culture, fatty acid-free BSA is useful, for researchers to be able fatty acids specific to their cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific FA′s to use in cell culture. The use of FA-free BSA also addresses concerns about endogenous fatty acids that might be potentially present in non-FA-free BSA.

Packaging

10, 50, 100 g in poly bottle

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Heat shock fractionated
  • Essentially fatty acid-free

Preparation Note

Prepared using heat shock fractionation. Further purified to reduce fatty acid content.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Ninette Genster et al.
Scientific reports, 7(1), 3852-3852 (2017-06-22)
Ficolins are a family of pattern recognition molecules that are capable of activating the lectin pathway of complement. A limited number of reports have demonstrated a protective role of ficolins in animal models of infection. In addition, an immune modulatory...
Ov D Slayden et al.
Contraception, 94(5), 521-526 (2016-10-25)
Intrauterine administration of polidocanol foam (PF) can create fallopian tube occlusion in nonhuman primates. The objective of this study was to determine if PF-induced tubal obstructions contain collagen in the extracellular matrix. We compared tissue samples of the intramural fallopian...
Marie Christou-Kent et al.
EMBO molecular medicine, 10(5) (2018-04-18)
The genetic causes of oocyte meiotic deficiency (OMD), a form of primary infertility characterised by the production of immature oocytes, remain largely unexplored. Using whole exome sequencing, we found that 26% of a cohort of 23 subjects with OMD harboured...
Improved in vitro culture conditions to enhance the survival, mitotic activity, and transplantability of rainbow trout type a spermatogonia
Shikina S and Yoshizaki G
Biology of Reproduction, 83(2), 268-276 (2010)
The role of inertial cavitation in acoustic droplet vaporization
Fabiilli ML, et al.
IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, 56(5), 1006-1017 (2009)
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