A3294

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, protease free, pH 7, ≥98%

Synonym(s):
Albumin bovine serum, Bovine albumin, BSA
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

Quality Level

biological source

bovine

assay

≥98%

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

application(s)

ELISA: suitable
blood typing: suitable
microbiological culture: suitable

impurities

protease, none detected

loss

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Albumin is abundantly present in human plasma. Increased levels of albumin result in dehydration. Albumin turnover is usually found in children with iron deficiency anemia. It is a predictive indicator for liver disease.

Application

Bovine Serum Albumin has been used:
  • to block non-specific binding of the antibodies
  • as a component of activating medium for inducing sperm motility
  • to determine the protein concentration of the bacteriocin by the bicinchoninic acid (BCA) method

Packaging

10, 50, 100, 500 g in poly bottle
1, 5 kg in poly drum

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Heat shock fractionated
  • Protease-free

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Leuconostoc carnosum 4010 has the potential for use as a protective culture for vacuum-packed meats: culture isolation, bacteriocin identification, and meat application experiments
Budde B B, et al.
International Journal of Food Microbiology, 83(2), 171-184 (2003)
Cryopreservation of Atlantic cod Gadus morhua L. spermatozoa: effects of extender composition and freezing rate on sperm motility, velocity and morphology
Butts I A E, et al.
Cryobiology, 61(2), 174-181 (2010)
Histomorphometric alteration and cell-type specific modulation of arylhydrocarbon receptor and estrogen receptor expression by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin and 17beta-estradiol in mouse experimental model of endometriosis
Kitajima M, et al.
Reproductive Toxicology, 18(6), 793-801 (2004)
Derek Toms et al.
Frontiers in bioengineering and biotechnology, 8, 534-534 (2020-06-26)
We have developed an accessible software tool (receptoR) to predict potentially active signaling pathways in one or more cell type(s) of interest from publicly available transcriptome data. As proof-of-concept, we applied it to mouse photoreceptors, yielding the previously untested hypothesis...
Kathrin S Heinz et al.
Nucleic acids research, 46(12), 6112-6128 (2018-05-12)
The replication of the genome is a highly organized process, both spatially and temporally. Although a lot is known on the composition of the basic replication machinery, how its activity is regulated is mostly unknown. Several chromatin properties have been...

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