05470

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, crystallized, ≥98.0% (GE)

Synonym(s):
Albumin bovine serum, Bovine albumin, BSA
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

Quality Level

biological source

bovine

assay

≥98.0% (GE)

form

lyophilized powder

mol wt

~66 kDa

packaging

glass bottle of

application(s)

cell culture | mammalian: suitable
electron microscopy: suitable

pH

4.8-7.5

solubility

H2O: slightly soluble

anion traces

chloride (Cl-): ≤2000 ppm
sulfate (SO42-): ≤1000 ppm

cation traces

Ca: ≤500 ppm
Cd: ≤5 ppm
Co: ≤5 ppm
Cr: ≤100 ppm
Cu: ≤20 ppm
Fe: ≤20 ppm
K: ≤50 ppm
Mg: ≤500 ppm
Mn: ≤5 ppm
Na: ≤7000 ppm
Ni: ≤5 ppm
Pb: ≤5 ppm
Zn: ≤10 ppm

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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Application

Bovine serum albumin has been used to quantify proteins in raw enzyme extracts. It has also been used as a blocking agent for mitochondrial imaging.
Albumin from bovine serum can be used for Electron and Immunoelectron microscopy. It can also be used for isolating and culturing neonatal rat cardiac ventricular myocytes.

Biochem/physiol Actions

Bovine serum albumin also refers as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also trigger insulin dependent diabetes mellitus.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Other Notes

Sales restrictions may apply
Review

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

In vitro digestibility of specific dsRNA by enzymes of digestive tract of shrimp Litopenaeus vannamei
Alvarez-Sanchez AR, et al.
Revista MVZ Cordoba, 22(2), 5867-5880 (2017)
Glucagon-like peptide-1 inhibits vascular smooth muscle cell dedifferentiation through mitochondrial dynamics regulation
Torres G, et al.
Biochemical Pharmacology, 104(1), 52-61 (2016)
T. Peters, Jr.
Adv. Prot. Chem., 37, 161-161 (1985)
Effect of dietary chitin on digestive enzyme activity, growth and survival of Macrobrachium tenellum juvenile prawns
Santos-Romero RB, et al.
Latin American Journal of Aquatic Research, 45(1), 130-138 (2017)
J D Marsh et al.
Circulation, 98(3), 256-261 (1998-08-11)
The role of androgens in producing cardiac hypertrophy by direct action on cardiac myocytes is uncertain. Accordingly, we tested the hypothesis that cardiac myocytes in adult men and women express an androgen receptor gene and that myocytes respond to androgens...

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