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T8787

Sigma-Aldrich

Triton X-100

for molecular biology

Synonym(s):
t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein:
2315025
MDL number:
PubChem Substance ID:
NACRES:
NA.21

Quality Level

grade

for molecular biology

description

non-ionic

form

liquid

mol wt

micellar avg mol wt 80,000
average mol wt 625

aggregation number

100-155

technique(s)

protein purification: suitable
protein quantification: suitable
western blot: suitable

pH

9.7

CMC

0.2-0.9 mM (20-25°C)

mp

6 °C

transition temp

cloud point 65 °C
pour point ~7 °C

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

density

1.06 g/mL at 25 °C (lit.)

HLB

13.5

application(s)

diagnostic assay manufacturing
hematology
histology

foreign activity

DNase and RNase, none detected

storage temp.

room temp

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton X-100 has been used:
  • In immunohistochemistry for staining the Flat-mount retinas
  • Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
  • To permeabilise cells during Immunofluorescent microscopic studies
  • As a positive control in LDH assay to determine the cell membrane integrity
  • For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
  • For the preparation of outer membrane protein exctract
  • As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells
  • In the treatment of tissue sections for Immunofluorescence labeling

Packaging

50, 100, 250 mL in poly bottle

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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