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Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture


Quality Level






cell culture | mammalian: suitable


endotoxin, tested


glucose: 1.0 g/L (Dextro)
phenol red: no
NaHCO3: 0.35 g/L

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General description

The essential function of Hanks′ Balanced Salt solution is to maintain pH as well as osmotic balance. It also provides water and essential inorganic ions to cells.
Formulation: click here.


Calcium and magnesium support cell adhesion. Used to minimize cell attachment or aggregation.
Hanks′ Balanced Salt solution has been used:

  • to study the degradation and cytotoxic effects of the composite material and the ion and/or magnetite release from the scaffolds.
  • to measure the stability of the magnetic scaffold material in bone tissue engineering.
  • in Fluorometric Measurements of intracellular calcium levels [Ca2+]
  • for isolating polymorphonuclear leukocytes (PMN) from umbilical cord blood
  • For assessing airway inflammation in mice

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when growing cells at low densities.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

A Tampieri et al.
Nanotechnology, 22(1), 015104-015104 (2010-12-08)
Magnetic bio-hybrid porous scaffolds have been synthesized, nucleating nano-apatite in situ on self-assembling collagen, in the presence of magnetite nano-particles. The magnetic phase acted as a sort of cross-linking agent for the collagen, inducing a chemico-physical-mechanical stabilization of the material
Andreas Koutroulis et al.
Scientific reports, 9(1), 19019-19019 (2019-12-15)
Tricalcium silicate (TCS)-based materials produce calcium hydroxide as a byproduct of their hydration reaction. The present study investigated whether calcium ion release (CIR) affects their biological and antimicrobial properties when used as pulp protection materials. The effect of incorporation of
N Bock et al.
Acta biomaterialia, 6(3), 786-796 (2009-10-01)
In recent years, interest in tissue engineering and its solutions has increased considerably. In particular, scaffolds have become fundamental tools in bone graft substitution and are used in combination with a variety of bio-agents. However, a long-standing problem in the
Iliana Moreschi et al.
The Journal of biological chemistry, 281(42), 31419-31429 (2006-08-24)
Micromolar concentrations of extracellular beta-NAD+ (NAD(e)+) activate human granulocytes (superoxide and NO generation and chemotaxis) by triggering: (i) overproduction of cAMP, (ii) activation of protein kinase A, (iii) stimulation of ADP-ribosyl cyclase and overproduction of cyclic ADP-ribose (cADPR), a universal
Janhavi Sharma et al.
Journal of immunological methods, 351(1-2), 30-35 (2009-10-06)
One of the initial steps in the inflammatory process involves the adherence and transmigration of circulating polymorphonuclear leukocytes (PMN) across the endothelial cell monolayer. One of the main constituents of the neutrophil phagosome that contributes to bacterial killing is myeloperoxidase


Human Astrocytes (HA) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Rat Dermal Fibroblasts (RDF) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Bovine Coronary Artery Endothelial Cells (BCAEC) Culture Protocol

Technical information for working with bovine brain microvascular endothelial cells including thawing, subculturing and cryopreservation

Rat Aortic Smooth Muscle Cells (RAOSMC) Culture Protocol

Technical information for working with rat aortic smooth muscle cells including thawing, subculturing and cryopreservation

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