Andrew C Tolonen et al.
Journal of visualized experiments : JoVE, (89)(89), doi:10-doi:10 (2014-07-22)
Stable isotope labeling of peptides by reductive dimethylation (ReDi labeling) is a method to accurately quantify protein expression differences between samples using mass spectrometry. ReDi labeling is performed using either regular (light) or deuterated (heavy) forms of formaldehyde and sodium...
Guangfa Shi et al.
Organic letters, 17(1), 38-41 (2014-12-18)
Direct trifluoromethylation of arenes using TFA as the trifluoromethylating reagent was achieved with Ag as the catalyst. This reaction not only provides a new protocol for aryl C-H trifluoromethylation, but the generation of CF3· from TFA may prove useful in...
Risto Kostiainen et al.
Journal of chromatography. A, 1216(4), 685-699 (2008-09-19)
The most widely used ionization techniques in liquid chromatography-mass spectrometry (LC-MS) are electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI). All three provide user friendly coupling of LC to MS. Achieving optimal LC-MS conditions is...
W C Mahoney et al.
The Journal of biological chemistry, 255(23), 11199-11203 (1980-12-10)
A method employing reverse phase high performance liquid chromatography has been developed for the purification of large denatured peptides. The cyanogen bromide fragments of hemoglobin alpha, beta, and gamma chains (13 to 91 residues in length) could be separated and...
Macro- and micromethods for the determination of serum vitamin A using trifluoroacetic acid.
J B NEELD et al.
The Journal of nutrition, 79, 454-462 (1963-04-01)