MilliporeSigma
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U1250

Sigma-Aldrich

Urea

ReagentPlus®, ≥99.5%, pellets

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Synonym(s):
Carbonyldiamide, Carbamide
Linear Formula:
NH2CONH2
CAS Number:
57-13-6
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
200-315-5
MDL number:
MFCD00008022
PubChem Substance ID:
57654718
NACRES:
NA.21

Quality Level

300

product line

ReagentPlus®

assay

≥99.5%

form

pellets

mp

132-135 °C (lit.)

solubility

H2O: 8 M

density

1.335 g/mL at 25 °C (lit.)

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

human ... CA1(759) , CA2(760)
rat ... Ppm1a(24666)

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General description

Urea is a chaotropic agent and is used for protein denaturation. It disturbs the hydrogen bonds in the secondary, tertiary and quaternary structure of proteins. Urea can also disturb hydrogen bonds present in DNA secondary structure.

Application

Urea has been used as a protein denaturing agent.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Packaging

1 kg in poly bottle
5 kg in poly drum

Legal Information

ReagentPlus is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

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Quotes and Ordering

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Protein and cell wall polysaccharide carbonyl determination by a neutral pH 2, 4-dinitrophenylhydrazine-based photometric assay.
Georgiou C D, et al.
Redox Biology, 17, 128-142 (2018)
Sheehan D.
Physical Biochemistry: Principles and Applications (2013)
Protein carbonyl determination by a rhodamine B hydrazide-based fluorometric assay.
Georgiou C D, et al.
Redox Biology, 17, 236-245 (2018)
Florin Sasarman et al.
Human molecular genetics, 24(10), 2841-2847 (2015-02-06)
Addition of the trinucleotide cytosine/cytosine/adenine (CCA) to the 3' end of transfer RNAs (tRNAs) is essential for translation and is catalyzed by the enzyme TRNT1 (tRNA nucleotidyl transferase), which functions in both the cytoplasm and mitochondria. Exome sequencing revealed TRNT1
Gurudayal et al.
Nano letters, 15(6), 3833-3839 (2015-05-06)
Photoelectrochemical water splitting half reactions on semiconducting photoelectrodes have received much attention but efficient overall water splitting driven by a single photoelectrode has remained elusive due to stringent electronic and thermodynamic property requirements. Utilizing a tandem configuration wherein the total
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Articles

Enzymatic Assay of Proteinase K with Hemoglobin Substrate

Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).

Protocols

Enzymatic Assay of Urease from Jack Beans

To standardize a procedure for the enzymatic assay of Urease, from Jack Beans

Enzymatic Assay of Proteinase K (EC 3.4.21.64)

Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).

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