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  • U1250
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U1250

Sigma-Aldrich

Urea

ReagentPlus®, ≥99.5%, pellets

Synonym(s):
Carbamide, Carbonyldiamide
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

Quality Level

product line

ReagentPlus®

Assay

≥99.5%

form

pellets

mp

132-135 °C (lit.)

solubility

H2O: 8 M

density

1.335 g/mL at 25 °C (lit.)

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

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1 of 4

This Item
1.04166U48831.08486
Urea ReagentPlus®, ≥99.5%, pellets

Sigma-Aldrich

U1250

Urea

Urea granulated, EMPROVE® EXPERT, Ph. Eur., BP, JP, USP, ACS

SAFC

1.04166

Urea

Urea 8 M (after reconstitution with 16 mL high purity water)

Supelco

U4883

Urea

Urea crystalline, EMPROVE® ESSENTIAL, Ph. Eur., BP, ChP, JP, USP

SAFC

1.08486

Urea

assay

≥99.5%

assay

-

assay

-

assay

98.0-102.0% (HPLC)

form

pellets

form

granular

form

powder

form

crystalline, solid

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

-

mp

132-135 °C (lit.)

solubility

H2O: 8 M

solubility

-

solubility

H2O: soluble (1 vial plus 16 mL)

solubility

1000 g/L

density

1.335 g/mL at 25 °C (lit.)

density

1.335 g/mL at 25 °C (lit.)

density

-

density

1.335 g/mL at 25 °C (lit.)

Quality Level

300

Quality Level

500

Quality Level

100

Quality Level

500

General description

Urea is a chaotropic agent and is used for protein denaturation. It disturbs the hydrogen bonds in the secondary, tertiary and quaternary structure of proteins. Urea can also disturb hydrogen bonds present in DNA secondary structure.

Application

Urea has been used as a protein denaturing agent.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Legal Information

ReagentPlus is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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