MilliporeSigma
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F7258

Sigma-Aldrich

Fast Green FCF

certified by the Biological Stain Commission

Synonym(s):
Food green 3
Linear Formula:
C37H34N2O10S3Na2
CAS Number:
Molecular Weight:
808.85
Colour Index Number:
42053
Beilstein:
5718212
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

dye content, ≥90%

mp

290 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O

InChI

1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2

InChI key

RZSYLLSAWYUBPE-UHFFFAOYSA-L

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This Item
465224F72521.04194
Fast Green FCF certified by the Biological Stain Commission

Sigma-Aldrich

F7258

Fast Green FCF

Sunset Yellow FCF Dye content 90 %

Sigma-Aldrich

465224

Sunset Yellow FCF

Fast Green FCF Dye content ≥85 %

Sigma-Aldrich

F7252

Fast Green FCF

grade

certified by the Biological Stain Commission

grade

-

grade

-

grade

-

form

powder

form

powder

form

powder

form

solid

solubility

H2O: 1 mg/mL

solubility

-

solubility

H2O: 1 mg/mL, clear

solubility

150 g/L

storage temp.

room temp

storage temp.

room temp

storage temp.

-

storage temp.

2-30°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

dye content, ≥90%

composition

Dye content, 90%

composition

Dye content, ≥85%

composition

Dye, ≥85% spectrophotometric assay

Application

Fast Green FCF has been used:
  • along with safranin for the staining of nodule sections
  • in the collagen deposit staining procedure
  • for the staining of protein and starch
  • as a dye to detect and inject DNA solution in chicken embryo
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Suitability

Certified for the safranin-fast green FCF stain for plant cells; as a substitute for aniline blue in Gomori′s one-step trichrome procedure.

Pictograms

Exclamation markHealth hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Muta. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

352.4 °F - closed cup

Flash Point(C)

178 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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