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11815016001

Roche

Anti-HA Affinity Matrix

from rat IgG1

Synonym(s):

affinity matrix, anti-ha

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About This Item

UNSPSC Code:
12352203

biological source

rat

Quality Level

conjugate

agarose conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3F10, monoclonal

form

slurry

packaging

pkg of 1 mL (settled resin volume)

manufacturer/tradename

Roche

isotype

IgG1

epitope sequence

YPYDVPDYA

capacity

2-8 nmol/mL binding capacity

storage temp.

2-8°C

General description

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal)

Specificity

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal).

Immunogen

Amino acids 98-106 from the human influenza virus hemagglutinin protein

Application

Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by:
  • Western blotting using the Anti-HA antibody
  • Silver staining (or similar protein stain)
  • In coimmunoprecipitation.
Use Anti-HA Affinity Matrix for:
  • Immunoprecipitation of HA-tagged proteins from mammalian, bacterial, and yeast cell extracts
  • Affinity column purification of HA-tagged proteins from crude protein extracts

Features and Benefits

  • Achieve enhanced specificity and reduced cross-reactivity of Anti-HA High Affinity.
  • Use nondenaturing conditions for elution of your HA-tagged protein of choice.
  • Purify even rarely expressed HA-tagged proteins.
  • Highly specific to YPYDVPDYA.
  • Suitable for purification of proteins containing HA-epitop as N-terminal, C-terminal or internal fusion.
  • Applicable with crued cell extracts from mammalian, bacterial and yeast expression systems.

Quality

Each lot of Anti-HA Affinity Matrix is tested for its ability to purify a HA-tagged protein expressed in stably transfected cells from a crude mammalian extract. The immunoprecipitate is analyzed by western blot using Anti-HA, High Affinity antibody.

Physical form

1 ml settled resin of Anti-HA Affinity Matrix in PBS containing 0.09% sodium azide (w/v); 2 ml suspension equals to 1 ml bed volume. One plastic column with top and bottom caps is included.

Analysis Note

Yield of 9 nmol purified protein/ml affinity matrix was determined using a whole-cell bacterial extract containing expressed HA-tagged bacterial alkaline phosphatase.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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