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11332465001

Sigma-Aldrich

TWEEN® 20

non-ionic, aqueous solution, 10% (w/v)

Synonym(s):

TWEEN 20, Tween, Tween 20

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About This Item

UNSPSC Code:
12352200

description

Formula: C58H114O26, for n = 20

form

solution

mol wt

Mr 1228 (for n= 20)

packaging

pkg of 5 × 10 mL

manufacturer/tradename

Roche

technique(s)

cell culture | plant: suitable
microbiological culture: suitable

CMC

~0.06 mM

absorption

= 0.025 at 254 nm at 0.05 % (w/v)

shipped in

wet ice

storage temp.

2-8°C

General description

Tween 20, also referred to as poly(oxyethylene)n-sorbitan-monolaurate, is a non-ionic surfactant. It is mainly used in membrane research.

Application

Tween 20 is used for:
  • solubilizing membrane proteins during isolation of membrane-protein complexes.
  • a subsequent protein detection by UV light absorption, due to its low UV absorbance.
  • ELISA (enzyme-linked immunosorbent assay) and immunoblots.

Quality

Purity: <2 ppm peroxide content (as H2O2), <200 μM carbonyls, <100 μS conductivity. Specially purified for membrane research.

Physical form

10% (w/v) aqueous solution under nitrogen, supplied in injection glass bottles.

Preparation Note

Working concentration: >10 mg/mg membrane lipid

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available

Certificates of Analysis (COA)

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Danny K Chou et al.
Journal of pharmaceutical sciences, 94(6), 1368-1381 (2005-04-29)
The objectives of this work were to determine the effects of nonionic surfactants (Tween 20 and Tween 80) on agitation-induced aggregation of the recombinant fusion protein, Albutropintrade mark (human growth hormone genetically fused to human albumin), and to characterize the

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