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P1754

Sigma-Aldrich

TWEEN® 80

viscous liquid

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Synonym(s):
TWEEN® 80, POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80
CAS Number:
9005-65-6
MDL number:
MFCD00082107
NACRES:
NA.21

description

non-ionic

Quality Level

200

form

viscous liquid

mol wt

micellar avg mol wt 79,000
average mol wt 1310

composition

Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

aggregation number

60

CMC

0.012 mM (20-25°C)

transition temp

cloud point 65 °C

density

1.064 g/cm3

HLB

15

application(s)

cell analysis

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

InChI key

RGPBUVUVZKQNHD-MDZDMXLPSA-N

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TWEEN® 80 viscous liquid

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P1754

TWEEN® 80

TWEEN® 80 from non-animal source

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P6224

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TWEEN® 80 for synthesis

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8.22187

TWEEN® 80

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P6349

TWEEN® 80

form

viscous liquid

form

viscous liquid

form

liquid

form

viscous liquid

mol wt

micellar avg mol wt 79,000, average mol wt 1310

mol wt

micellar avg mol wt 79,000, average mol wt 1310

mol wt

-

mol wt

average mol wt ~1310

aggregation number

60

aggregation number

60

aggregation number

-

aggregation number

-

CMC

0.012 mM (20-25°C)

CMC

0.012 mM (20-25°C)

CMC

-

CMC

-

transition temp

cloud point 65 °C

transition temp

cloud point 65 °C

transition temp

flash point >149 °C

transition temp

-

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
TWEEN® 80 has been used in drug vehicle solution for specific injections in mice and rats. It has been used for the determination of esterase activity in bacterial cells.

Biochem/physiol Actions

TWEEN® 80 is a polysorbate surfactant which helps in stabilizing proteins and in protein membrane studies. It is regularly used for the extraction of membrane proteins and isolation of nuclei from cells.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

235.4 °F - closed cup

Flash Point(C)

> 113 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Pathogenic factors of Pseudomonas cepacia isolates from patients with cystic fibrosis.
Gessner AR and Mortensen JE
Journal of Medical Microbiology, 33, 115-115 (1990)
Zandra Gerdes et al.
PloS one, 14(2), e0205378-e0205378 (2019-02-20)
The role of microplastic (MP) as a carrier of persistent organic pollutants (POPs) to aquatic organisms has been a topic of debate. However, the reverse POP transport can occur if relative contaminant concentrations are higher in the organism than in
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Langmuir : the ACS journal of surfaces and colloids, 32(49), 13009-13019 (2016-12-14)
Therapeutic proteins are privileged in drug development because of their exquisite specificity, which is due to their three-dimensional conformation in solution. During their manufacture, storage, and delivery, interactions with material surfaces and air interfaces are known to affect their stability.
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