Immobilon ECL Ultra Western HRP Substrate

Western blotting, HRP


Quality Level


western blot: suitable

detection method


shipped in


General description

Immobilon® ECL Ultra Western HRP Substrate is an ultra-sensitive reagent with long signal duration for the detection of horseradish peroxidase (HRP)-labeled antibodies on western blots. The intense signal created permits sensitive detection at the mid-to-low femtogram range using x-ray film or digital imaging systems.


Western blotting, HRP
"Immobilon® ECL Ultra substrate is compatible with both PVDF andnitrocellulose blotting membranes, as well as commonly used buffers and blocking reagents.

Sufficient for 5000 cm²"


Solution A- 250 mL

Solution B- 250 mL

Storage and Stability

Store at 2–8 °C

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.
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