MilliporeSigma
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IEVH00005

Millipore

Immobilon®-E PVDF Membrane

1 roll, 26.5 cm x 1.875 m, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.

Synonym(s):
Anti-CVID3, Streptavidin-AP, Anti-IMD27B
eCl@ss:
32031602
NACRES:
NB.23

material

PVDF membrane
plain filter
white filter

Quality Level

feature

easily wetted with water

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

filter L × W

26.5 cm × 1.875 m

pore size

0.45 μm pore size

compatibility

for use with Amido black
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Transillumination

detection method

chemiluminescent
colorimetric
radioactive

shipped in

ambient

General description

Immobilon-E is the first PVDF membrane for Western blotting that can be wetted with with aqueous transfer buffer. This eliminates the alcohol pre-wet step required with other PVDF membranes.
Filter Code: IEVH
Primary Binding Mechanisms: Electrostatic, hydrophobic

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificate of Analysis

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Certificate of Quality

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Articles

Semi-Dry or Tank Electrotransfer Troubleshooting

The possible causes and potential remedies for poor or inconsistent transfer of proteins from a gel to the Western blot membrane. Optimal transfer conditions may vary depending on the molecular weight of the protein of interest.

Protocols

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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