IEVH85R

Immobilon^®-E PVDF Membrane

The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.

• Synonym(s): Rabbit Anti-PH20 (C-terminal), Mouse Anti-T-cell surface antigen T3/Leu-4 epsilon chain, Mouse Monoclonal Anti-T3E, Rabbit Anti-HYAL3, Rabbit Anti-TYH
• eCl@ss: 32031613
• NACRES: NB.23

Properties

• material: PVDF membrane; plain filter; white filter
• feature: easily wetted with water
• manufacturer/tradename: Immobilon^®
• technique(s): western blot: suitable
• filter L × W: 8.5 cm × 10 m
• pore size: 0.45 μm pore size
• compatibility: for use with Amido black; for use with Colloidal gold; for use with Coomassie brilliant blue; for use with India ink; for use with Ponceau-S red; for use with Sypro<TMSYMBOL></TMSYMBOL> ruby; for use with Transillumination
• detection method: chemiluminescent; colorimetric; radioactive
• shipped in: ambient

Description

General description

This 8.5cm x 10m roll offers 1-cut convenience for standard mini-blots (7 x 8.5cm) and midi-blots (8.5 x 13.5cm). It works with the optional Immobilon NOW Dispenser (catalog number IMDISP).

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa
- Compatible with chemiluminescent and chromogenic detection

Immobilon^® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes.

Features & Benefits:
- High protein binding provides stronger signal
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be stripped and reprobed multiple times

Immobilon-E is the first PVDF membrane for Western blotting that can be wetted with with aqueous transfer buffer. This eliminates the alcohol pre-wet step required with other PVDF membranes.

Filter Code: IEVH

Primary Binding Mechanisms: Electrostatic, hydrophobic

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3