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IEVH85R

Millipore

Immobilon®-E PVDF Membrane

The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.

Synonym(s):
Rabbit Anti-PH20 (C-terminal), Mouse Anti-T-cell surface antigen T3/Leu-4 epsilon chain, Mouse Monoclonal Anti-T3E, Rabbit Anti-HYAL3, Rabbit Anti-TYH
eCl@ss:
32031613
NACRES:
NB.23

material

PVDF membrane
plain filter
white filter

feature

easily wetted with water

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

filter L × W

8.5 cm × 10 m

pore size

0.45 μm pore size

compatibility

for use with Amido black
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Transillumination

detection method

chemiluminescent
colorimetric
radioactive

shipped in

ambient

General description

This 8.5cm x 10m roll offers 1-cut convenience for standard mini-blots (7 x 8.5cm) and midi-blots (8.5 x 13.5cm). It works with the optional Immobilon NOW Dispenser (catalog number IMDISP).

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa
- Compatible with chemiluminescent and chromogenic detection

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes.

Features & Benefits:
- High protein binding provides stronger signal
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be stripped and reprobed multiple times
Immobilon-E is the first PVDF membrane for Western blotting that can be wetted with with aqueous transfer buffer. This eliminates the alcohol pre-wet step required with other PVDF membranes.
Filter Code: IEVH
Primary Binding Mechanisms: Electrostatic, hydrophobic

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificate of Analysis

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Certificate of Quality

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Articles

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Protocols

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Hand-casting gels for PAGE and SDS-PAGE using TurboMix™ Bis-Tris Gel Casting Kits

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix™ Bis-Tris Gel Casting Kits

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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