Immobilon Western Chemiluminescent HRP Substrate

Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.


storage conditions

2 to 8 °C

Quality Level


pkg of 250 mL Luminol
pkg of 250 mL Peroxide solution




western blot: suitable

membrane area

5000  cm2 , membrane coverage


peroxidase conjugate

detection method


shipped in

wet ice


For use on immunoblots.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Nicolas Bidère et al.
Nature, 458(7234), 92-96 (2009-01-02)
The transcription factor NF-kappaB is required for lymphocyte activation and proliferation as well as the survival of certain lymphoma types. Antigen receptor stimulation assembles an NF-kappaB activating platform containing the scaffold protein CARMA1 (also called CARD11), the adaptor BCL10 and...
Elizabeth I Vink et al.
Virology, 422(2), 317-325 (2011-11-30)
The adenovirus E4-ORF3 protein promotes viral replication by relocalizing cellular proteins into nuclear track structures, interfering with potential anti-viral activities. E4-ORF3 targets transcriptional intermediary factor 1 alpha (TIF1α), but not homologous TIF1β. Here, we introduce TIF1γ as a novel E4-ORF3-interacting...
Reinhard David et al.
The European journal of neuroscience, 31(6), 978-993 (2010-04-10)
Nicotinic acetylcholine receptors (nAChRs) mediate fast synaptic transmission in ganglia of the autonomic nervous system. Here, we determined the subunit composition of hetero-pentameric nAChRs in the mouse superior cervical ganglion (SCG), the function of distinct receptors (obtained by deletions of...
Novel irreversible epidermal growth factor receptor inhibitors by chemical modulation of the cysteine-trap portion.
Carmi, Caterina, et al.
Journal of medicinal chemistry, 53, 2038-2050 (2010)
The possible causes and potential remedies for challenges encountered dot/slot blotting, which uses vacuum filtration to transfer protein onto a microporous membrane.
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A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.
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This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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