SCR005

Sigma-Aldrich

Accutase cell detachment solution

A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

eCl@ss:
32160801

Quality Level

form

liquid

manufacturer/tradename

Chemicon®

input

sample type cardiac stem cell(s)
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)

shipped in

dry ice

Related Categories

General description

A cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including SmartPlastic. ACCUTASE does not contain mammalian or bacterial derived products.

Because the reagent does not contain universally proteolytic enzymes it may not release all cell/tissue types uniformly. Some optimization of cell type and passage number, as well as, incubation time and reagent strength typically needs to be performed to optimize the reagents effectiveness.

ACCUTASE has been shown to be effective on a wide variety of cell types including: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.

Each lot of ACCUTASE is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.

Application

Cell Detachment

1. Thaw ACCUTASE® at room temperature.

2. Wash plate, flask or beads with sterile PBS.

3. Add ACCUTASE to culture dish or flask using aseptic procedures at 10 ml per 75-cm2 surface area.

4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).

5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required



Optimal incubation times and reagent strength needs to be determined for each cell/tissue type tested by the enduser.

Physical form

100 ml, ready to use, frozen sterile liquid. 1X ACCUTASE enzymes in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5 mM EDTA·4Na and 3 mg/L Phenol Red.

Storage and Stability

Stable when stored at -20°C. Refer to lot expiration date. Recommended storage upon receipt is -20°C. After thawing, ACCUTASE may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMP

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

HDAC1 regulates pluripotency and lineage specific transcriptional networks in embryonic and trophoblast stem cells.
Kidder B. L. & Palmer S.
Nucleic Acids Research null
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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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