Autophagy in lung cancer.

Methods in enzymology (2009-02-17)
Jerry J Jaboin, Misun Hwang, Bo Lu
ABSTRACT

Lung cancer is the leading cause of cancer-related deaths worldwide. The relatively poor cure rate in lung cancer patients has been associated with a resistance to chemotherapy and radiation that is at least in part related to defects in cellular apoptotic machinery. Exploitation of another form of cell death, autophagy, has the capacity to improve the therapeutic gain of current therapies. In an effort to develop novel treatment strategies to enhance the therapeutic ratio for lung cancer, we wish to better understand the role of autophagic cell death for the sensitization of lung cancer. This text reviews the most up to date protocols and techniques for the study of autophagic cell death in lung cancer models. Others may use these techniques as a framework for study within their experimental models.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Accutase cell detachment solution, A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.
Sigma-Aldrich
Anti-von Willebrand Factor Antibody, Chemicon®, from rabbit
Sigma-Aldrich
Triton X-100, non-ionic, aqueous solution