C5914

Sigma-Aldrich

Cell Dissociation Solution Non-enzymatic 1x

Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

product line

BioReagent

form

solution

concentration

1 ×

application(s)

cell culture | mammalian: suitable

pH

6.5-7.5

shipped in

wet ice

storage temp.

2-8°C

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Related Categories

General description

Cell dissociation by non-enzymatic methods in human mesenchymal stromal cells aid in their detachment and preserves their functionality especially the expression of cell surface chemokine receptor.

Application

Cell Dissociation Solution Non-enzymatic 1x has been used for the dissociation of retinal astrocytes culture, U87 glioblastoma tumor cells and cardiac stem cells (CSCs).
Special non-enzymatic formulation for gently dislodging adherent cell types from plastic or glass surfaces.

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  2. Can one use Product C5914, Cell Dissociation Solution, with tissue?

    We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.

  3. Does Product C5914, Cell Dissociation Solution, contain EDTA?

    Yes, it does contain EDTA; the concentration is proprietary.

  4. When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?

    No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.

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