MilliporeSigma
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MPSTD3

Millipore

mPAGE® Unstained Protein Standard

form

liquid

mol wt

10-200 kDa

packaging

pkg of 500 μL

storage temp.

−20°C

General description

The mPAGE® Unstained Protein Standard is a mixture of twelve recombinant unstained proteins ranging from 10kDa to 200kDa. The 85kDa, 25kDa, and 10kDa bands have higher intensity to serve as reference points. mPAGE® Unstained Protein Standard is suitable for molecular weight determination after SDS-PAGE or Western blotting and serves as a standard to calibrate the mobility of prestained markers.

Storage Buffer: 20 mM Tris-phosphate(pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol. Load 2.5 to 10 μl per lane.

  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Application

SDS-PAGE, Western blot
  • Immunoblotting (Western)
  • Western Blotting

Other Notes

4 °C for up to 3 months, or 25 °C for up to two weeks.

Legal Information

MPAGE is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Protocols

Hand-casting gels for PAGE and SDS-PAGE using TurboMix™ Bis-Tris Gel Casting Kits

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix™ Bis-Tris Gel Casting Kits

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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