Kit for Molecular Weights 14,000-500,000 Non-denaturing has been used as a standard for non-denaturing PAGE (polyacrylamide gel electrophoresis) analysis. It has also been used to calibrate columns for gel exclusion chromatography.
The kit contains 5 vials of proteins (1 mg of each) having a molecular weight range of 14 - 545 kDa and a technical bulletin.
α-Lactalbumin from bovine milk
Carbonic Anhydrase from bovine erythrocytes
Albumin from chicken egg white
Albumin from bovine serum
Urease from Jack bean
Reconstitute the contents of each vial of protein marker with 1ml of water, with the exception of Urease, Catalog Number U7752. Reconstitute the vial of Urease with 0.5 ml of water and mix until the protein is in solution. Then add 0.5 ml of glycerol to the Urease solution to stabilize the protein during freeze thaw cycles. Solutions may be stored at -20 °C for future use. It is recommended that repeated freezing and thawing be avoided. Stock solutions may be dispensed into working aliquots, frozen, and then discarded after 2-3 uses. Immediately before use, thaw an aliquot of each prepared protein marker and dilute with an equal volume of sample buffer.