MABF3035

Anti-Chikungunya virus E1 Antibody, clone CHK-166

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: CHK-166, monoclonal
• mol wt: calculated mol wt 138.7 kDa
• purified by: using protein G
• species reactivity: virus
• packaging: antibody small pack of 100 μL
• technique(s): ELISA: suitable; inhibition assay: suitable; neutralization: suitable
• isotype: IgG2κ
• epitope sequence: Unknown
• UniProt accession no.: Q5XXP3
• shipped in: dry ice
• target post-translational modification: unmodified

Description

General description

Chikungunya virus (CHIKV) that causes distinctive polyarthritis or polyarthralgia with clinical features such as fever, maculopapular rash, and myalgia is transmitted by Aedes spp of mosquitoes. Immune compromised individuals may face serious complications, including encephalitis and mortality. CHIKV genome contains a single-stranded positive-sense RNA that encodes four non-structural proteins known as nsP1, nsP2, nsP3, and nsP4 and also five structural proteins that include a capsid protein, three envelope glycoproteins known as (E1, E2, and E3, and a small molecule known as 6K. The mature alphavirus particles express E1 and E2 heterodimers that form 80 trimeric spikes on the surface of the virion. The mature E2 protein contains three domains with immunoglobulin-like folds: N-terminal domain A, domain B at the tip; and the C-terminal domain C located proximal to the viral membrane. The ectodomain E1 protein consists of three domains known as D1, DII, and DIII. The DIII domain is an immunoglobulin-like domain connected to D1 and DII by a flexible linker. Both E1 and E2 proteins are responsible for virus entry into host cells. The E2 glycoprotein interacts with a cellular receptor, resulting in the virus internalization and the E1 glycoprotein mediates virus fusion to host cell under low pH conditions. Following the fusion of the viral envelope with the endosomal membrane, the viral genomic RNA is released into the cytoplasm and starts replicating. Clone CHK-166 is a moderately inhibitory antibody that protects Ifr -/- mice from lethal infection and when used with clone CHK-152 it provides viral resistance and extends the treatment window by up to 36 hours post infection. (Ref.: Zhang, R., et al. (2019). Cell Reports 28(10); 2647-2658; Pal, P., et al. (2013). PLoS Pathog. 9(4); e1003312).

Specificity

Clone CHK-166 is a mouse monoclonal antibody that detects E1 protein of Chikungunya virus. It targets an epitope within the DII region of E1 protein, adjacent to the fusion loop.

Immunogen

Irf-7 deficient mice injected with La Reunion 2006 OPY-1 strain of Chikungunya virus (CHIKV-LR) and boosted with CHIKV virus-like particles.

Application

Quality Control Testing

Evaluated by ELISA with recombinant Chikungunya Virus E1 protein.

ELISA Analysis: Various dilutions of this antibody detected recombinant Chikungunya virus E1 protein.

Tested Applications

Inhibition Analysis: A representative lot inhibited CHIKV infection when added after virus adsorption to the cell surface. (Pal, P., et al. (2013). PLoS Pathog. 9(4):e1003312).

ELISA Analysis: A representative lot detected Chikungunya virus E1 in ELISA applications (Zhang, R., et al. (2019). Cell Rep. 28(10): 2647-2658.e5).

Neutralizing Analysis: A representative lot neutralized CHIKV and protected immunocompromised mice against disease. (Pal, P., et al. (2013). PLoS Pathog. 9(4):e1003312).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Chikungunya virus E1, clone CHK-166, Cat. No. MABF3035, is a mouse monoclonal antibody that detects Chikungunya virus (CHIKV) E1 protein and is tested for use in ELISA, Inhibition, and Neutralizing assay.

Physical form

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable