MABF3024
Anti-Mayaro Virus E1 Antibody, clone MAY-115
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
MAYV-115, monoclonal
mol wt
calculated mol wt 137.06 kDa
purified by
using protein G
species reactivity
virus
packaging
antibody small pack of 100 μL
technique(s)
ELISA: suitable
neutralization: suitable
isotype
IgG2κ
epitope sequence
N-terminal extracellular domain
UniProt accession no.
shipped in
2-8°C
target post-translational modification
unmodified
General description
Structural polyprotein (UniProt: Q8QZ72; also known as p130) is encoded by the MAYVgp3 gene (Gene ID: 935141) in Mayaro virus. Following its synthesis it is cleaved into the following six chains: Capsid protein (aa 1-258), Precursor of protein E3/E2 (aa 259-746), Assembly protein E3 (aa 259-324), Spike glycoprotein E2 (aa 325-746), 6K protein (aa 747-806), and Spike glycoprotein E1 (aa 807-1242). Mayaro virus (MAYV) is a mosquito-transmitted arthritogenic alphavirus in the Togaviridae family of positive-stranded RNA viruses. Its envelope glycoproteins associate in the endoplasmic reticulum and form a heterotrimer comprised of the E3, E2, and E1 proteins. E2 is a single-pass type I membrane protein that plays a role in viral attachment to target host cell, by binding to the cell receptor. It is synthesized as a p62 precursor (precursor of protein E3/E2), which is then processed by furin at the cell membrane just before virion budding, giving rise to E2-E1 heterodimer. The p62-E1 heterodimer is shown to be stable, whereas E2-E1 is unstable and dissociates at low pH. It has been reported that p62 is processed at the last step, presumably to avoid E1 fusion activation before its final export to cell surface. The C-terminus region of E2 contains a transitory transmembrane region that is disrupted by palmitoylation and results in reorientation of the C-terminal tail from lumenal to cytoplasmic side. Clone MAY-115 offers protection against lethal effects of MAYV in a murine model when injected one day prior to virus inoculation. However, its protective effects are significantly reduced when injected two days post infection. (Ref.: Earnest, JT., et al. (2019). J. Exp. Med. 216(10); 2282-2301).
Specificity
Clone MAYV-115 is a mouse monoclonal antibody that detects E1 glycoprotein in Mayaro virus.
Immunogen
Mayaro virus, CH strain and boosted with recombinant MAYV E2 protein mixed with incomplete Freund′s adjuvant.
Application
Quality Control Testing
Evaluated by ELISA with recombinant MAYV E1 protein.
ELISA Analysis: Various dilutions of this antibody detected recombinant E1 protein of Mayaro virus CH (strain Peru).
Tested Applications
Neutralizing Analysis: A representative lot neutralized Mayarao virus and protected mice from lethal virus challenge. (Earnest, J.T., et al. (2019). J Exp Med.;216(10):2282-2301).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by ELISA with recombinant MAYV E1 protein.
ELISA Analysis: Various dilutions of this antibody detected recombinant E1 protein of Mayaro virus CH (strain Peru).
Tested Applications
Neutralizing Analysis: A representative lot neutralized Mayarao virus and protected mice from lethal virus challenge. (Earnest, J.T., et al. (2019). J Exp Med.;216(10):2282-2301).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Mayaro Virus E1, clone MAY-115 , Cat. No. MABF3024, is a mouse monoclonal antibody that detects Mayaro virus E1 glycoprotein and is tested for use in ELISA and Neutralizing Activity.
Physical form
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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