Recommended Products
biological source
rat
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
12F9, monoclonal
mol wt
calculated mol wt 165.14 kDa
observed mol wt ~165 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100 μg
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
epitope sequence
N-terminal
Protein ID accession no.
UniProt accession no.
shipped in
2-8°C
target post-translational modification
unmodified
Gene Information
human ... ASXL1(171023)
General description
Polycomb group protein ASXL1 (UniProt: Q8IXJ9; also known as Additional sex combs-like protein 1) is encoded by the ASXL1 (also known as KIAA0978) gene (Gene ID: 171023) in human. ASXL1 is a member of the ASXL family that is expressed at low levels in various tissues. However, higher expression is reported in testes. It is a member of the ASXL family with a highly conserved ASX homology (ASXH) domain at the N-terminal region and a plant homeodomain (PHD) finger (aa 1503-1540) at the C-terminal region. The ASXH domain mediates its interaction with a partner protein BAP1, which is an essential component of the polycomb repressive deubiquinase complex (PR-DUB), in which it is involved in deubiquitination of monoubiquitinated histone H2A at lysine 119 (H2AK119ub). ASXL1 also acts as a sensor of N6-methyladenosine methylation on DNA (m6A). It recognizes and binds m6A DNA, leading to its ubiquitination and degradation by TRIP12, thereby inactivating the PR-DUB complex and regulating polycomb silencing. Its HARE-HTH-type domain (aa 11-86) recognizes and binds m6A. Mutations in ASXL1 gene are known to perturb development and cause Bohring-Opitz syndrome that is characterized by severe intrauterine growth retardation, poor feeding, and mental retardation. Some mutations are also observed in myeloid neoplasms, including myelodysplastic syndromes, chronic myelomonocytic leukemia, and acute myeloid leukemia and are associated with poor prognosis. (Ref.: Matheus, F., et al. (2019). Stem Cell Reports 12(5); 861-868; Asada, S., et al. (2018). Nat. Commun. 9: Article 2733; Scheuermann, JC., et al. (2010). Nature. 465(7295); 243-247).
Specificity
Clone 12F9 is a rat monoclonal antibody that detects human Polycomb group protein ASXL1.
Immunogen
His-tagged recombinant fragment corresponding to the first 618 amino acids from the N-terminal region of human Polycomb group protein ASXL1.
Application
Quality Control Testing
Evaluated by Western Blotting in Human spleen tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ASXL1 in Huma spleen tissue lysate.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected ASXL1 in Immunocytochemistry applications (Matheus, F.. et al. (2019). Stem Cell Reports. 12(5):861-868).
Western Blotting Analysis: A representative lot detected ASXL1 in Western Blotting applications (Matheus, F.. et al. (2019). Stem Cell Reports. 12(5):861-868).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human spleen tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ASXL1 in Huma spleen tissue lysate.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected ASXL1 in Immunocytochemistry applications (Matheus, F.. et al. (2019). Stem Cell Reports. 12(5):861-868).
Western Blotting Analysis: A representative lot detected ASXL1 in Western Blotting applications (Matheus, F.. et al. (2019). Stem Cell Reports. 12(5):861-868).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-ASXL1, clone 12F9, Cat. No. MABE1933, is a rat monoclonal antibody that detects ASXL1 and is tested for use in Immunocytochemistry and Western Blotting.
Physical form
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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