dot blot: suitable
western blot: suitable
26.5 cm × 1.875 m
0.2 μm pore size
262 μg/cm2 (insulin)
340 μg/cm2 adsorption capacity (BSA)
448 μg/cm2 adsorption capacity (goat IgG)
for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination
10-13 - German Storage Class 10 to 13
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Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.
The possible causes and potential remedies for poor or inconsistent transfer of proteins from a gel to the Western blot membrane. Optimal transfer conditions may vary depending on the molecular weight of the protein of interest.