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ISEQ00005

Millipore

Immobilon® -PSQ PVDF Membrane

1 roll, 27 cm x 1.875 m, 0.2 µm pore size, transfer membrane for Western blotting

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.23

product name

Immobilon®-PSQ PVDF Membrane, 1 roll, 27 cm x 1.875 m, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

material

PVDF membrane
plain filter
white filter

Quality Level

400

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

27 cm × 1.875 m

pore size

0.2 μm pore size

capacity

262 μg/cm2 (insulin)
 340 μg/cm2 adsorption capacity (BSA)
 448 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
radioactive

shipped in

ambient

General description

Filter Code: ISEQ
Primary Binding Mechanisms: Electrostatic, hydrophobic

Application

1 roll, 27 cm x 1.875 m, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Protein Visualization

Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.

Semi-Dry or Tank Electrotransfer Troubleshooting

The possible causes and potential remedies for poor or inconsistent transfer of proteins from a gel to the Western blot membrane. Optimal transfer conditions may vary depending on the molecular weight of the protein of interest.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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