MilliporeSigma
All Photos(1)

AB1211

Sigma-Aldrich

Anti-β Galactosidase Antibody

Chemicon®, from rabbit

eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

vertebrates, E. coli

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

Gene Information

human ... GLB1(2720)

Specificity

Recognizes Beta-Galactosidase (E. coli). Monospecific by IEP. Some cross reactivity with Beta Galactosidase from other species may occur.

Immunogen

Beta-Galactosidase (E. coli).

Application

Anti-β Galactosidase Antibody is an antibody against β Galactosidase for use in ELISA, Immunohistochemistry, Immunoprecipitation, Western Blotting.
ELISA: 1:10,000-1:50,000 when assayed against 1 μg of Beta Galactosidase in a standard sandwich ELISA.

Immunoblotting: 1:2000-1:10,000 on beta-galactosidase fusion proteins.

Immunohistochemistry: 1:1,000-1:5000 on tissue fixed with 4% paraformaldehyde.

Immunoprecipitation

Optimal working dilutions must be determined by end user.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Physical form

Ammonium sulfate precipitation and DEAE-cellulose chromatography
Format: Purified
Immunoglobulin Purified by delipidation, salt fractionation and ion-exchange chromatography. Liquid in 0.02M Potassium Phosphate buffer, 0.15M Sodium Chloride, with 0.01% sodium azide.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Proteins tagged with beta galactosidase

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service