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Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Anti Rat, Anti Rat Hrp
MDL number:

biological source


Quality Level


peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies




buffered aqueous solution


direct ELISA: 1:10,000

shipped in

dry ice

storage temp.


Related Categories

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG conjugated to a detectable substrate is a useful tool for the analysis of target proteins. Anti-Rat IgG (whole molecule)-Peroxidase antibody binds all rat immunoglobulins.
IgG (immunoglobulin γ) is a monomeric antibody containing a γ Fc region as a part of heavy chain, which is linked to the Fab arm through a hinge region. It is the most abundant serum antibody and constitutes to 70% of the total immunoglobulins.


Purified rat IgG


Anti-Rat IgG (whole molecule)-Peroxidase antibody is suitable for use in ELISA and immunoblot at 1:5000 dilutions.
Lysates generated from primary cells isolated from lymphoid organs of P. monodon shrimp were analyzed by western blot using HRP-conjugated goat anti-rat as the secondary antibody at a 1:4000 dilution.


1 mL in glass bottle

Biochem/physiol Actions

IgG (immunoglobulin γ), upon epitope binding, mediates the activation of classical complement pathway. Interaction between the host cell membrane and IgG antibody results in type II hypersensitivity reaction. Decreased serum levels of IgG contributes to common variable immunodeficiency, thus making the individual easily prone to infections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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