58346-M

Tetracycline, Hydrochloride

CAS 64-17-5 is a broad-based antibiotic agent that blocks protein synthesis by inhibiting binding of aminoacyl tRNA to the A-site of ribosomes. Induces cold shock-response and enhances P450 expression in bacteria.

• Synonym(s): Tetracycline, Hydrochloride
• Empirical Formula (Hill Notation): C₂₂H₂₄N₂O₈
• CAS Number: 64-75-5
• Molecular Weight: 444.43
• MDL number: MFCD00078142
• UNSPSC Code: 51284043

Properties

• Quality Level: 200
• form: crystalline solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• concentration: ≥900 μg/mg
• color: yellow
• solubility: water: 20 mg/mL; ethanol: 5 mg/mL
• storage temp.: 10-30°C
• InChI: 1S/C22H24N2O8.ClH/c1-21(31)8-5-4-6-11(25)12(8)16(26)13-9(21)7-10-15(24(2)3)17(27)14(20(23)30)19(29)22(10,32)18(13)28;/h4-6,9-10,15,25-26,30-32H,7,23H2,1-3H3;1H/b20-14-;/t9-,10-,15-,21+,22-;/m0./s1
• InChI key: DYQBMRZOOXYHIM-NCKOGSTASA-N

Description

General description

An antibiotic that inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit, preventing binding of aminoacyl tRNA to the A-site and blocking translocation. Used commonly for selection of the bacterial tet^r gene whose product, a 399-amino acid membrane-associated protein, functions in active export of the drug from the cell.

Broad-based antibiotic agent that blocks protein synthesis by inhibiting binding of aminoacyl tRNA to the A-site of ribosomes. Induces cold shock-response and enhances P450 expression in bacteria.

Biochem/physiol Actions

Primary Target
binding of aminoacyl tRNA to the A-site of ribosomes

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for 1 month at -20°C. Agar plates are stable for 2 weeks at 4°C. Autoclaved media should be cooled to ≤55°C before adding tetracycline.

Other Notes

Kusano, K., et al. 1999. Arch. Biochem. Biophys. 367, 129.
Lech, K. and Brent, R. 1995. In Current Protocols in Molecular Biology, Vol. 1 (Ausubel, F.M., et al. eds.) John Wiley & Sons, New York Unit 1.4.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York, p. 1.6, A.6.
Chopra, I. and Howe, T.G.B. 1978. Microbiol. Rev. 42, 707.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08,GHS07,GHS09
• signalword: Warning
• hcodes: H315,H319,H335,H361fd,H410
• pcodes: P202 - P261 - P273 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable