529659

DDD00033325

≥95% (HPLC), syrup, single hESC survival promoter, Calbiochem^®

• Synonym(s): Pro-survival Compound, DDD00033325, DDD00033325
• Empirical Formula (Hill Notation): C₁₇H₁₉N₅
• CAS Number: 1259034-82-6
• Molecular Weight: 293.37
• UNSPSC Code: 51111800
• NACRES: NA.77

Properties

• product name: Pro-survival Compound, DDD00033325, A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background with equal or higher potency than Y-27632.
• Quality Level: 100
• assay: ≥95% (HPLC)
• form: syrup
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: DMSO: 100 mg/mL
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: C12=CC=NN1C=CC(N[C@@H]3CCN(CC4=CC=CC=C4)C3)=N2

Description

General description

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC₅₀ = 3.9 M), with equal or higher potency than Y-27632 (Cat. No. 688000) in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 M. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (>83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 M. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 M than Y-27632 without marked adverse effects for up to 120 hours.

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC₅₀ = 3.9 M), with equal or higher potency than Y-27632 in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 M. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (>83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 M. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 M than Y-27632 without marked adverse effects for up to 120 hours.

Packaging

Packaged under inert gas

Warning

Toxicity: Regulatory Review (Z)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Andrews, P.D., et al. 2010. Biochem. J.432, 21.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable