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52332

Sigma-Aldrich

Phenylmethylsulfonyl Fluoride - CAS 329-98-6 - Calbiochem

Phenylmethylsulfonyl Fluoride, CAS 329-98-6, is an irreversible inhibitor of serine proteases. It causes sulfonylation of the active-site serine residues.

Synonym(s):
Benzylsulfonyl Fluoride, PMSF, Phenylmethylsulfonyl Fluoride - CAS 329-98-6 - Calbiochem
Empirical Formula (Hill Notation):
C7H7FO2S
CAS Number:
Molecular Weight:
174.19
MDL number:
NACRES:
NA.77

Quality Level

assay

≥99% (GC)

form

crystalline solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

white to off-white

solubility

ethanol: soluble
isopropanol: soluble
methanol: soluble

shipped in

ambient

storage temp.

10-30°C

InChI

1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2

InChI key

YBYRMVIVWMBXKQ-UHFFFAOYSA-N

General description

Irreversible inhibitor of serine proteases. Its mechanism of action is analogous to that of diisopropylfluorophosphate. PMSF causes sulfonylation of the active-site serine residues. Also reported to inhibit internucleosomal DNA fragmentation in immature thymocytes. For a related, more stable inhibitor, see AEBSF (Cat. No. 101500).
Irreversible inhibitor of serine proteases. Its mechanism of action is analogous to that of diisopropylfluorophosphate. PMSF causes sulfonylation of the active-site serine residues. Also reported to inhibit internucleosomal DNA fragmentation in immature thymocytes. Effective at a concentration of 50 µM.

Packaging

1 g in Alu drum
5 g in Glass bottle
25 g in Fibre case

Biochem/physiol Actions

Product does not compete with ATP.
Reversible: no
Primary Target
serine proteases
Cell permeable: no

Warning

Toxicity: Corrosive (L)

Reconstitution

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 9 months at 4°C.
t½ = 1 hour at pH 7.5. Effective concentration: 50 μM.

Other Notes

Seitz, R., et al. 1993. Int. J. Cancer53, 514.
Weaver, V.M., et al. 1993. Biochem. Cell. Biol.71, 488.
Bourgain, R.H., et al. 1992. Adv. Exp. Med. Biol.316, 427.
Chang, C.T., et al. 1992. Biochem. Int.28, 707.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

CorrosionSkull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Oral - Skin Corr. 1B

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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