499050

Osteocalcin, Bovine Bone

• Synonym(s): Osteocalcin, Bovine Bone, Bone-Gla-Protein, BGP
• Empirical Formula (Hill Notation): C₂₆₃H₃₇₂N₆₆O₈₂S₂
• Molecular Weight: 5834.29
• UNSPSC Code: 12352202
• NACRES: NA.42

Properties

• assay: ≥98% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• pI: 4.0 - 4.5
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Single chain vitamin K-dependent protein produced by osteoblasts. Osteocalcin purified from bovine bone. Binds to phospholipid vesicles in the presence of Ca²⁺ (K_d = 6 µM). Also binds hydroxylapatite. A good marker of bone turnover. May play a role in bone mineralization, bone resorption, and bone formation.

Single chain vitamin K-dependent protein produced by osteoblasts. Osteocalcin purified from bovine bone. Binds to phospholipid vesicles in the presence of Ca²⁺ (K_d = 6 µM). Also binds hydroxylapatite. Possible role in bone mineralization, bone resorption, and bone formation. Note: Gla corresponds to γ-carboxyglutamic acid.

Warning

Toxicity: Standard Handling (A)

Sequence

H-Tyr-Leu-Asp-His-Trp-Leu-Gly-Ala-Pro-Ala-Pro-Tyr-Pro-Asp-Pro-Leu-Gla-Pro-Lys-Arg-Gla-Val-Cys²³-Gla-Leu-Asn-Pro-Asp-Cys²⁹-Asp-Glu-Leu-Ala-Asp-His-Ile-Gly-Phe-Gln-Glu-Ala-Tyr-Arg-Arg-Phe-Tyr-Gly-Pro-Val-OH (disulfide bond: 23 → 29)

Physical form

In 75 mM NaCl, 10 mM sodium phosphate buffer, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Carter, S.D., et al. 1996. J. Anim. Sci. 74, 2719.
Watson, K.E., et al. 1994. J. Clin. Invest.93, 2106.
Tracy, R.P., et al. 1990. J. Bone Min. Res.5, 451.
Gendreau, M.A., et al. 1989. J. Biol. Chem.264, 6972.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable