407951

Ionomycin, Free Acid, Streptomyces conglobatus in Solution

• Synonym(s): Ionomycin, Free Acid, Streptomyces conglobatus in Solution
• Empirical Formula (Hill Notation): C₄₁H₇₂O₉
• CAS Number: 56092-81-0
• Molecular Weight: 709.00
• MDL number: MFCD06798385
• UNSPSC Code: 12352106
• NACRES: NA.76

Properties

• assay: ≥90% (HPLC)
• Quality Level: 100
• form: solution
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: clear colorless to slight yellow
• shipped in: wet ice
• storage temp.: −20°C
• InChI: 1S/C41H72O9/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47)/b13-11+,34-24-/t25-,26-,27+,28-,29-,30+,31+,32-,33?,36+,37?,39-,40+,41+/m1/s1
• InChI key: PGHMRUGBZOYCAA-OJFQOPKISA-N

Description

General description

Highly specific for divalent cations (Ca²⁺ > Mg²⁺ >> Sr²⁺ = Ba²⁺). More effective than antibiotic A23187 as a mobile ion carrier for Ca²⁺. Complexes with Ca²⁺ between pH 7 and 9.5, causing significant absorption in the UV range; however, in contrast to A23187 (Cat. No. 100105), it is not fluorescent. Useful for studies of Ca²⁺ transport across biological membranes and measurement of cytoplasmic free Ca²⁺. Induces apoptotic neuronal degeneration in embryonic cortical neurons. Also induces cell cycle arrest in the G₁ interval of mature Burkitt lymphoma cell lines.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Mobile ion carrier for Ca2+.

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Physical form

A 5 mM (1 mg/282 µl) solution of Ionomycin, Free Acid, Streptomyces conglobatus (Cat. No. 407950) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Aagaard-Tillery, K.M., and Jelinek, D.F. 1995. J. Immunol.155, 3297.
Takei, N., and Endo, Y. 1994. Brain Res. 652, 65.
Dupuy d’Angeac, A., et al. 1993. J. Cell Physiol.154, 44.
Lee, H.C. 1993. J. Biol. Chem.268, 293.
Kuznetsov, G., et al. 1992. J. Biol. Chem. 267, 3932.
Toeplitz, B.K., et al. 1979. J. Am. Chem. Soc.101, 3344.
Liu, C.M., and Hermann, T.E. 1978. J. Biol. Chem.253, 5892.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: 188.6 °F - closed cup - (Dimethylsulfoxide)
• flash_point_c: 87 °C - closed cup - (Dimethylsulfoxide)