389561

Hyaluronidase, Streptomyces hyaluronlyticus nov. sp.

• Synonym(s): Hyaluronidase, Streptomyces hyaluronlyticus nov. sp.
• CAS Number: 37326-33-3
• Enzyme Commission number: 3.2.1.35 (BRENDA, IUBMB)
• MDL number: MFCD00131351
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• conjugate: conjugate (Glucosaminoglycan)
• Quality Level: 100
• form: lyophilized powder
• specific activity: ≥2000 units (A280)
• packaging: ampule of ≥100 U (1 U = 1 TRU (Turbidity Reducing Unit))
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: 0.5 M acetate, pH 6.0: 30 U/mL; water: 30 U/mL
• foreign activity: protease ≤0.05 U/ampule (pH 6.0)
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Native hyaluronidase from Streptomyces hyaluronlyticus nov. species. Catalyzes the degradation of hyaluronic acid. Produces δ4,5-unsaturated tetra- and hexasaccharides.

Native hyaluronidase from Streptomyces hyaluronlyticus nov. species. Catalyzes the degradation of hyaluronic acid. Produces δ4,5-unsaturated tetra- and hexasaccharides.



Please Note: The appearance of this product can vary from gray cottony fibers to balled up droplets with no change in quality.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One TRU (Turbidity Reducing Unit = 1 U) is defined as the amount of enzyme that will hydrolyze 50% of hyaluronic acid, determined by a 50% reduction in turbidity, in 30 min at 60°C pH 6.0.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions prepared in water are stable for up to 2 weeks at 4°C and stock solutions prepared in 500 mM acetate buffer, pH 6.0, are stable for up to 1 month at 4°C.

Other Notes

Baker, J.R., et al. 2002. Biochem. J.365, 317.
Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable