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H3884

Sigma-Aldrich

Hyaluronidase from bovine testes

Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid

Synonym(s):
Hyaluronoglucosaminidase, Hyaluronate 4-glycanohydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

Quality Level

biological source

bovine testis

type

Type IV-S

form

lyophilized powder (essentially salt-free)

specific activity

750-3000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

UniProt accession no.

application(s)

diagnostic assay manufacturing

relevant disease(s)

cancer

storage temp.

−20°C

Gene Information

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Related Categories

Packaging

50, 100, 500 mg in poly bottle
1 g in poly bottle

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will cause a change in % transmittance at 600 nm of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 mL reaction mixture (45 minute assay).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Articles

Hepatocyte Culture

Hepatocytes are epithelial cells found in the liver. They perform important functions, such as helping to detoxify blood, and to synthesize transport proteins, such as lipoprotein, albumin and transferrin. Primary and secondary cultures of hepatocytes are useful for studying the mechanisms of liver regeneration and differentiation.

Carbohydrate Analysis III

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

This procedure may be used for Hyaluronidase products.

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