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H6254

Sigma-Aldrich

Hyaluronidase from sheep testes

Type V, lyophilized powder, ≥1,500 units/mg solid

Synonym(s):
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

sheep testis

Quality Level

type

Type V

form

lyophilized powder

specific activity

≥1,500 units/mg solid

mol wt

55 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

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General description

Hyaluronidase is present in high amount in testis as it aids fertilization of the mammalian egg. Mammalian hyaluronidase has a catalytic epidermal growth factor (EGF)-like domain and a C-terminal cysteine rich region.

Application

Hyaluronidase has been used:
  • as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules
  • in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry
  • in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines

Packaging

500 mg in poly bottle
1 g in poly bottle

Biochem/physiol Actions

Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively.
These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will cause a change in % transmittance at 600 nm of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 mL reaction mixture (45 minute assay).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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