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Sigma-Aldrich

H2A.X Phosphorylation Assay Kit (Flow Cytometry)

The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X.

eCl@ss:
32161000
NACRES:
NA.32

Quality Level

manufacturer/tradename

Upstate®

application(s)

activity assay: suitable
flow cytometry: suitable

NCBI accession no.

UniProt accession no.

detection method

fluorometric

shipped in

dry ice

Related Categories

General description

Phosphorylation of the histone variant H2A.X is a rapid and sensitive response to double strand DNA breaks. The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated histone H2A.X.

The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. Cells are cultured in microplates, treated with agents that induce DNA damage or apoptosis, which stimulates H2A.X phosphorylation. Cells are then fixed and permeabilized in preparation for staining and detection. Histone H2A.X phosphorylated at serine 139 is detected by the addition of the anti-phospho-Histone H2A.X, FITC conjugate. Cells are then scanned in a flow cytometer to quantitate the number of cells staining positive for phosphorylated Histone H2A.X.

Application

Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Packaging

100 assays

Components

Anti-phospho H2A.X, FITC conjugate

Normal Mouse IgG, FITC conjugate (Cat.# 12-487)

16X Fixation Solution

10X Permeabilization Solution

10X Wash Solution

Quality

phosphorylation of Histone H2A.X

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Hongfeng Yuan et al.
The Journal of biological chemistry, 285(7), 5085-5096 (2009-12-17)
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Sarah A Krueger,George D Wilson,Evano Piasentin,Michael C Joiner,Brian Marples
International Journal of Radiation Oncology, Biology, Physics null
Yu Wang et al.
Mutation research, 734(1-2), 20-29 (2012-05-09)
Berberine has been shown to possess anti-tumor activity against a wide spectrum of cancer cells. It inhibits cancer cell proliferation by inducing cell cycle arrest, at G1 and/or G2/M, and apoptosis. While it has been documented that berberine induces G1...
Erica K Benson et al.
Journal of cell science, 123(Pt 15), 2605-2612 (2010-07-08)
Hutchinson-Gilford Progeria Syndrome (HGPS) is a premature-aging syndrome caused by a dominant mutation in the gene encoding lamin A, which leads to an aberrantly spliced and processed protein termed progerin. Previous studies have shown that progerin induces early senescence associated...

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