MilliporeSigma
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R8000

Sigma-Aldrich

D-Ribulose 1,5-Diphosphate Carboxylase from spinach

partially purified powder, 0.01-0.1 unit/mg solid

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Synonym(s):
3-Phospho-D-glycerate carboxy-lyase(dimerizing), Rubisco
CAS Number:
9027-23-0
Enzyme Commission number:
4.1.1.39 (BRENDA, IUBMB)
MDL number:
MFCD00130908
NACRES:
NA.54

biological source

spinach

Quality Level

200

form

partially purified powder

specific activity

0.01-0.1 unit/mg solid

mol wt

557 kDa

storage temp.

−20°C

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D-Ribulose 1,5-Diphosphate Carboxylase from spinach partially purified powder, 0.01-0.1 unit/mg solid

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R8000

D-Ribulose 1,5-Diphosphate Carboxylase from spinach

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Glycerol 3-phosphate Oxidase from Pediococcus sp. lyophilized powder, 40-80 units/mg solid, pH 8.1

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G9637

Glycerol 3-phosphate Oxidase from Pediococcus sp.

biological source

spinach

biological source

-

biological source

-

biological source

bacterial (Pediococcus spp.)

form

partially purified powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

specific activity

0.01-0.1 unit/mg solid

specific activity

≥15 units/mg solid

specific activity

≥50 units/mg protein

specific activity

40-80 units/mg solid, pH 8.1

mol wt

557 kDa

mol wt

-

mol wt

93 kDa

mol wt

~76 kDa (gel filtration)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) amounts to 50% of the total spinach leaves associated soluble protein.
Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.
pH optimum: ~7.9.
KM for CO2: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.

Application

D-Ribulose 1,5-Diphosphate Carboxylase from spinach has been used:
  • as a test protein in pepsin digestion studies
  • as an innocuous or non-hazardous protein sample to test its effect on human intestinal epithelial cell lines
  • in isothermal titration calorimetry (ITC), and radiolabeled binding assays with abscisic acid

Biochem/physiol Actions

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) depends on Rubisco activase and chaperones for activation. It participates in plant photorespiration events by catalyzing the carboxylation and oxygenation of ribulose-1,5-bisphosphate. Abscisic acid inhibits the carboxylation activity of Rubisco.

Unit Definition

One unit will convert 1.0 μmole of D-RuDP and CO2 to 2.0 μmoles of D-3-phosphoglycerate per min at pH 7.8 at 25°C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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