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R8000
D-Ribulose 1,5-Diphosphate Carboxylase from spinach
partially purified powder, 0.01-0.1 unit/mg solid
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Synonym(s):
3-Phospho-D-glycerate carboxy-lyase(dimerizing), Rubisco
CAS Number:
MDL number:
NACRES:
NA.54
biological source
spinach
Quality Level
form
partially purified powder
specific activity
0.01-0.1 unit/mg solid
mol wt
557 kDa
storage temp.
−20°C
General description
D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) amounts to 50% of the total spinach leaves associated soluble protein.[23]
Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.
pH optimum: ~7.9.
KM for CO2: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.
pH optimum: ~7.9.
KM for CO2: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.
Application
D-Ribulose 1,5-Diphosphate Carboxylase from spinach has been used:
as an innocuous or non-hazardous protein sample to test its effect on human intestinal epithelial cell lines [76]in isothermal titration calorimetry (ITC), and radiolabeled binding assays with abscisic acid [70]
- as a test protein in pepsin digestion studies
Packaging
1 unit in poly bottle
5 units in poly bottle
Biochem/physiol Actions
D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) depends on Rubisco activase and chaperones for activation.[88] It participates in plant photorespiration events by catalyzing the carboxylation and oxygenation of ribulose-1,5-bisphosphate.[23] Abscisic acid inhibits the carboxylation activity of Rubisco.[70]
Unit Definition
One unit will convert 1.0 μmole of D-RuDP and CO2 to 2.0 μmoles of D-3-phosphoglycerate per min at pH 7.8 at 25°C.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificate of Analysis
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Certificate of Origin
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