07-2050
Anti-DNMT3A2 Antibody
from rabbit
Synonym(s):
DNA (cytosine-5-)-methyltransferase 3 alpha, DNA MTase HsaIIIA, DNA cytosine methyltransferase 3 alpha, DNA cytosine methyltransferase 3A2, DNA methyltransferase HsaIIIA
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
technique(s)
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... DNMT3A(1788)
General description
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. DNMT3 is a developmentally regulated DNA methyltransferase. DNMT3A2 is a second isoform of DNMT3 that is expressed from an alternative promoter. DNMT3A2 localizes to euchromatin and is thought to function in de novo methylation, rather than maintenance methylation. Binding of DNMT3L (DNMT3-like protein) to DNMT3A2 promotes a dramatic reorganization of DNMT3A2 subunits and leads to the formation of specific complexes with enhanced DNA methyltransferase activity. DNMT3A2 targets endogenous DNMT3L to embryonic stem cell chromatin and induces regional DNA methylation.
Specificity
Other homologies: Predicted to react with Mouse and Rat based on 100% sequence homology
The antibody recognizes isoform 2 of human DNMT3A. Additional lower molecular weight bands are detected in some samples.
Immunogen
Epitope: C-terminus
The immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human DNMT3A2.
Application
ELISA:
This antibody was shown by an outside laboratory to be suitable for ELISA.
Immunocytochemistry:
This antibody detects DMNT3A2 in the nuclei of NIH/3T3, HeLa, and A431 cells.
This antibody was shown by an outside laboratory to be suitable for ELISA.
Immunocytochemistry:
This antibody detects DMNT3A2 in the nuclei of NIH/3T3, HeLa, and A431 cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Use Anti-DNMT3A2 Antibody (Rabbit Polyclonal Antibody) validated in ICC, ELISA, WB to detect DNMT3A2 Antibody also known as DNA (cytosine-5)-methyltransferase 3 alpha.
Quality
Western Blot Analysis: 1:1,000 dilution of this antibody detected DNMT3A2 on 10 µg of L6 lysate.
Target description
~80 kDa
Physical form
Format: Purified
Protein A chromatography
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
L6, NIH/3T3, HeLa, or A431 cells.
L6, NIH/3T3, HeLa, or A431 cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Michael S Kareta et al.
The Journal of biological chemistry, 281(36), 25893-25902 (2006-07-11)
The DNMT3-like protein, DNMT3L, is required for germ line DNA methylation, although it is inactive as a DNA methyltransferase per se. Previous studies have shown that DNMT3L physically associates with the active de novo DNA methyltransferases, DNMT3A and DNMT3B, and
Keisuke Nimura et al.
Genes to cells : devoted to molecular & cellular mechanisms, 11(10), 1225-1237 (2006-09-27)
DNA methylation is involved in fundamental cellular processes such as silencing of genes and transposable elements, but the underlying mechanism of regulation of DNA methylation is largely unknown. DNA methyltransferase 3-like protein (Dnmt3L), a member of the Dnmt3 family of
Taiping Chen et al.
The Journal of biological chemistry, 277(41), 38746-38754 (2002-07-26)
Previous studies have shown that the Dnmt3b gene encodes multiple variants via alternative splicing. However, only one form of Dnmt3a has been identified to date. We report here the discovery of a small form of Dnmt3a, denoted Dnmt3a2, from both
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