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Key Documents

T6424

Sigma-Aldrich

胰蛋白酶 来源于人类胰腺

salt-free, lyophilized powder, vial of ≥1,000 BAEE units

同義詞:

Serine Protease 1

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

human pancreas

品質等級

形狀

salt-free, lyophilized powder

包裝

vial of ≥1,000 BAEE units

UniProt登錄號

儲存溫度

−20°C

基因資訊

相關類別

一般說明

胰蛋白酶分子有两个结构域:一个与酶的活性位点和色氨酸残基有关;另一种与 8-苯胺萘-1-磺酸结合有关。
胰蛋白酶是一种丝氨酸蛋白酶。胰腺可产生前体胰蛋白酶原并将其分泌到肠中。肠粘膜中的肠激酶可将胰蛋白酶原转化为胰蛋白酶。

應用

胰蛋白酶已被用于一项评估戊型肝炎病毒和人类星状病毒之间的相似性的研究。在一项研究中也使用了胰蛋白酶来表征培养原代成人上皮祖细胞或干细胞的一种独特技术。

單位定義

使用BAEE作为底物,一个BAEE单元将在pH7.6,25℃下产生每分钟0.001的A253。 反应体积= 3.2mL(1cm光程)。

數量

至少 1,000 个 BAEE 单位/瓶

分析報告

通过 E1%/280 确定的蛋白质。

其他說明

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


分析證明 (COA)

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Nature protocols, 16(7), 3522-3546 (2021-06-06)
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條款

This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).

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