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B4500

Sigma-Aldrich

Nα-苯甲酰基-L-精氨酸乙酯 盐酸盐

trypsin substrate, chromogenic, ≥97% (HPLC), powder

同義詞:

BAEE

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About This Item

經驗公式（希爾表示法）:
C15H22N4O3 · HCl
CAS號碼:
2645-08-1
分子量：:
342.82
Beilstein:
3781694
EC號碼:
220-157-0
MDL號碼:
MFCD00012579
分類程式碼代碼:
12352204
PubChem物質ID:
24891777
NACRES:
NA.32

產品名稱

Nα-苯甲酰基-L-精氨酸乙酯 盐酸盐, trypsin substrate

品質等級

200

化驗

≥97% (HPLC)

形狀

powder

溶解度

water: 50 mg/mL, clear, colorless

儲存溫度

2-8°C

SMILES 字串

Cl[H].CCOC(=O)[C@H](CCCNC(N)=N)NC(=O)c1ccccc1

InChI

1S/C15H22N4O3.ClH/c1-2-22-14(21)12(9-6-10-18-15(16)17)19-13(20)11-7-4-3-5-8-11;/h3-5,7-8,12H,2,6,9-10H2,1H3,(H,19,20)(H4,16,17,18);1H/t12-;/m0./s1

InChI 密鑰

HIXDELXKSSLIKB-YDALLXLXSA-N

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一般說明

Nα-苯甲酰基-L-精氨酸乙酯盐酸盐是胰蛋白酶底物。是用于光谱测定的非蛋白质底物。

應用

  • N

  • α-苯甲酰基-L-精氨酸乙酯盐酸盐作为底物，用于：
  • 在牙龈卟啉单胞菌（ P. gingivalis ）囊泡中测定肽基精氨酸脱亚氨酶（PPAD）
  • 测定番木瓜植物部位酶提取物的蛋白水解活性
  • 测定大鼠尾肌腱束的胰蛋白酶活性

基底

胰蛋白酶的原型底物。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
BCCH1622
0000135115
0000118817
0000118818
0000115477

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Maria T Dulay et al.
Analytical chemistry, 77(14), 4604-4610 (2005-07-15)
Trypsin is covalently linked to a photopolymerized sol-gel monolith modified by incorporating poly(ethylene glycol) (PSG-PEG) for on-column digestion of N(alpha)-benzoyl-l-arginine ethyl ester (BAEE) and two peptides, neurotensin and insulin chain B. The coupling of the enzyme to the monolith is
The mechanical, structural, and compositional changes of tendon exposed to elastase
Grant TM, et al.
Annals of Biomedical Engineering, 43(10), 2477-2486 (2015)
Weijun Wang et al.
Scientific reports, 9(1), 13630-13630 (2019-09-22)
Cellulases play important roles in the dietary fibre digestion in pigs, and have multiple industrial applications. The porcine intestinal microbiota display a unique feature in rapid cellulose digestion. Herein, we have expressed a cellulase gene, p4818Cel5_2A, which singly encoded a
Reynaldo Villalonga et al.
Biotechnology and bioengineering, 81(6), 732-737 (2003-01-17)
Bovine pancreatic trypsin was modified by the mono-6-amino-6-deoxy derivatives of alpha-, beta-, and gamma-cyclodextrin through a transglutaminase-catalyzed reaction. The trypsin-cyclodextrin conjugates, containing about 3 mol of oligosaccharide per mole of protein, were tested for their catalytic and stability properties. The
Mohammad K Hossain et al.
Colloids and surfaces. B, Biointerfaces, 181, 85-93 (2019-05-28)
Electric current responsive magnetic composite particles are prepared in three steps. In the first step, spherical and mesoporous submicrometer-sized magnetic iron oxide (Fe3O4) core particles are prepared by solvothermal method. Then magnetic Fe3O4 particles are functionalized with amine groups using
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文章

Papain

Papain is a cysteine protease of the peptidase C1 family. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

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Enzymatic Assay of Trypsin Inhibitor

Enzymatic Assay of Trypsin Inhibitor

Procedure for Enzymatic Assay of Trypsin (EC 3.4.21.4)

This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).

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