推薦產品
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
胰蛋白酶是一种丝氨酸蛋白酶，可特异性水解赖氨酸及精氨酸残基的羧基侧的多肽键。该款修饰后的胰蛋白酶经过甲苯磺酰基-L-氨基联苯氯甲基酮 (TPCK) 处理，可使外源性糜蛋白酶失活。每个包装含8个小瓶，每瓶含25 μg。
應用
TPCK处理的胰蛋白酶已被用于:
- 作为杜尔贝科改良伊格尔培养基（DMEM）的补充剂，用于上皮样猪肾细胞系（LLC-PK1） 的猪Delta冠状病毒（PDCoV）感染实验
- 分离人脐静脉内皮细胞（HUVEC） 用于膜联蛋白-V/碘化丙啶 (PI) 染色测定
- 用于多循环复制动力学的最小必需培养基 （MEM）
生化／生理作用
N-对-对甲苯磺酰基-L-苯丙氨酸氯甲基酮（TPCK） 可用作糜蛋白酶的不可逆抑制剂。胰蛋白酶诱导人纤维细胞分化。胰蛋白酶被广泛用于蛋白质组学中的蛋白质样品消化。在细胞培养中，进行胰蛋白酶消化是为了使贴壁细胞彼此分开并离开培养容器壁。
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
客戶也查看了
Zdeněk Perutka et al.
Molecules (Basel, Switzerland), 23(10) (2018-10-17)
Trypsin is the protease of choice for protein sample digestion in proteomics. The most typical active forms are the single-chain β-trypsin and the two-chain α-trypsin, which is produced by a limited autolysis of β-trypsin. An additional intra-chain split leads to
Jian-Ping Dai et al.
International journal of molecular sciences, 19(4) (2018-03-24)
Oxymatrine (OMT) is a strong immunosuppressive agent that has been used in the clinic for many years. In the present study, by using plaque inhibition, luciferase reporter plasmids, qRT-PCR, western blotting, and ELISA assays, we have investigated the effect and
Wentao Li et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(22), E5135-E5143 (2018-05-16)
Porcine deltacoronavirus (PDCoV), identified in 2012, is a common enteropathogen of swine with worldwide distribution. The source and evolutionary history of this virus is, however, unknown. PDCoV belongs to the Deltacoronavirus genus that comprises predominantly avian CoV. Phylogenetic analysis suggests
Yu Bai et al.
Microbial pathogenesis, 150, 104645-104645 (2020-12-08)
Influenza virus is responsible for significant morbidity and mortality worldwide. Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is the major cause of death in influenza virus infected patients. Recent studies indicated that active glucagon like peptide-1 (GLP-1) encoded by
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務