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Merck
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重要文件

4352157

Sigma-Aldrich

Trypsin, TPCK-Treated

同義詞:

6- (1-tosylamido-2-phenyl) ethyl chloromethyl ketone treated Trypsin, Modified trypsin (TPCK-Treated), Trypsin, TPCK treated

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.56

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

Trypsin is a serine protease that specifically hydrolyzes peptide bonds at the carboxyl side of Lysine and Arginine residues. This modified trypsin has been treated with N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) to inactivate extraneous chymotryptic activity. This trypsin product also contains calcium chloride, which may improve consistency in digestion. Each package contains 10 vials, with 25 μg in each vial. 

應用

Trypsin, TPCK-Treated has been used for the digestion of protein samples to generate peptides for LC-MS (liquid chromatography–mass spectrometry)/MS analysis.
Trypsin, TPCK-Treated has been used in enzymatic treatment to cleave the recombinant native B-cell lymphoma-extra large (Bcl-xL)-ΔTM monomer to generate fibrils (the third type of Bcl-xL fibril, called “BclxLcf37”). It has also been used in influenza infection experiments as a supplement in infection/DMEM serum-free medium to infect A549 cells with influenza A H1N1 virus at a multiplicity of infection (MOI) to perform multi-round infection.

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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