T3194

Tetanus toxin

from Clostridium tetani, cleaves synaptobrevin (VAMP-2)

• CAS號碼: 676570-37-9
• MDL號碼: MFCD01095768
• 分類程式碼代碼: 12352200
• NACRES: NA.77

屬性

• 產品名稱: 破伤风毒素 来源于破伤风梭菌,
• 分子量: 150 kDa (heavy chain (100 kDa) and a light chain (50 kDa) connected through an interchain disulfide bond)
• 品質等級: 100
• 溶解度: H₂O: soluble
• 儲存溫度: 2-8°C
• 基因資訊: Clostridium tetani E88 ... tetX(1061100)

描述

一般說明

Tetanus toxin is also known as tetanospasmin. It is a 150-kDa protein, produced by Clostridium tetani. This protein consists of a light and a heavy chain.

應用

Tetanus toxin from Clostridium tetani has been used as the exocytosis inhibitor/Golgi-dependent secretion to examine whether secretory carrier membrane protein (SCAMP5) stimulated α-synuclein secretion via the conventional Golgi-dependent pathway. It has also been used in peripheral blood mononuclear cells (PBMCs) culture for T cell proliferation screening.

Tetanus toxin from Clostridium tetani has been used to inhibit vesicular release in Pannexin 1-transfected Neuro2A cells¹.

生化／生理作用

Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals. Internalized by receptor-mediated endocytosis and carried to spinal cord and brain by transsynaptic retrograde transport. Blocks the release of glycine from inhibitory interneurons of the spinal cord. Cleaves synaptobrevin (VAMP-2), and blocks synaptic vesicle exocytosis in vivo.

Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals; blocks the release of glycine from inhibitory interneurons of the spinal cord; cleaves synaptobrevin (VAMP-2); blocks synaptic vesicle exocytosis in vivo.

Tetanus toxin can cause spastic paralysis.

準備報告

Purified from cultures of Clostridium tetani by a modification of the method of Robinson, et al.

重構

Each vial, when reconstituted with 100 μL of sterile purified water, contains 25 μg of tetanus toxin in 20 mM HEPES, pH 7.4 with 1.25% lactose.

分析報告

Two bands by SDS-PAGE under reducing conditions (100 kDa heavy chain and 50 kDa light chain).

安全資訊

• 象形圖: GHS06,GHS08
• 訊號詞: Danger
• 危險聲明: H300 + H330,H370
• 防範說明: P260 - P264 - P270 - P271 - P284 - P304 + P340 + P310
• 危險分類: Acute Tox. 1 Oral - Acute Tox. 2 Inhalation - STOT SE 1
• 標靶器官: Central nervous system
• 儲存類別代碼: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges