跳轉至內容
Merck
全部照片(2)

Key Documents

View All Documentation

S1816

Sigma-Aldrich

SYBR® Green JumpStart™ Taq ReadyMix™ 用于定量PCR，毛细管配制

SYBR® Green qPCR reagent for Roche LightCycler® capillary systems

登入查看組織和合約定價
全部照片(2)

About This Item

分類程式碼代碼:
41106300
NACRES:
NA.55

形狀

liquid

用途

sufficient for 100 reactions
 sufficient for 400 reactions

特點

dNTPs included
hotstart

濃度

1 units/reaction (20 μL reaction volume)

技術

qPCR: suitable

顏色

colorless

輸入

purified DNA

相容性

for use with Roche LightCycler 480

檢測方法

SYBR® Green

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

SYBR® Green JumpStart™ Taq ReadyMix™预混液（毛细管配方）结合了热启动酶JumpStart Taq的优点，是专门设计用于毛细管仪器（例如Roche LightCycler®实时热循环仪）的2x浓缩预混液。 SYBR Green JumpStart Taq ReadyMix是一种优化配方，包含SYBR Green I染料、JumpStart Taq DNA聚合酶、99%纯脱氧核苷酸、缓冲液和稳定剂。

SYBR Green Taq ReadyMix可用于单个产品实时扩增实验，也可用于评价产生荧光标记探针之前的引物序列。 不建议将荧光标记探针与SYBR Green I染料一起使用。

應用

SYBR® Green JumpStart™ Taq ReadyMix™预混液（适合定量PCR，毛细管专用）可用于实时定量聚合酶链式反应(RT-qPCR)和逆转录PCR(RT-PCR)。

特點和優勢

  • 方便的2×浓缩预混液，专门设计用于毛细管仪器，例如Roche LightCycler®，是高通量应用的理想选择
  • 提高特异性&目标产量-JumpStart™ Taq聚合酶可防止非特异性产物，从而获得更准确的CT值并改善定量
  • 该预混液可确保反应间一致性
  • 缩短了制备时间，并降低了多个移液步骤造成的污染风险
  • SYBR® Green I荧光染料特异性结合双链DNA，低价、易用、灵敏，是任意DNA序列定量分析的理想选择

包裝

提供一管25 mM MgCl2，以便于优化QPCR反应。
默认反应体积为20 μL

100RXN包装为1 X 1 mL
400RXN包装为1 X 4 mL

原則

SYBR Green I是一种常用的荧光DNA结合染料，可结合所有双链DNA，并通过测量整个循环中荧光的增加来监测检测。SYBR Green I最大激活和发射峰值分别在494nm和521nm。在加入一种热启动介导tag聚合酶，JumpStart Taq后，Sigma′s基于SYBR QPCR检测的特异性被增强。

JumpStart Taq抗体在室温下能够使DNA聚合酶失活。当温度在第一步循环变性中升高到70℃以上，复合体解离，聚合酶完全被激活。JumpStart Taq DNA聚合酶防止非特异性扩增，能够获得更为准确的CT值。

为制备反应，将10 μL ReadyMix加入引物、模板和水中，使最终反应体积为20 μL。

單位定義

一个单位在74℃下可在30分钟内将10 nmol总dNTP融入酸可沉淀DNA中。

法律資訊

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,994,056 and 6,171,785.. 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的，仅此等数量的产品用于购买者内部研究用途的的免责许可。我们未明确表示、暗示或以禁止反言的形式授予您其他专利声明下的任何权利（如美国专利号6,814,934号下的设备和系统专利声明）、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
JumpStart is a trademark of Sigma-Aldrich Co. LLC
LightCycler is a registered trademark of Roche
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

相關產品

產品號碼
描述
訂價

S4438

SYBR® Green JumpStart™ Taq ReadyMix™, for quantitative PCR, MgCI2 in buffer

S5193

SYBR® Green JumpStart™ Taq ReadyMix™, for quantitative PCR, without MgCl2 in buffer

S9194

高通量 QPCR 用 SYBR® Green JumpStart™ Taq ReadyMix™, SYBR® Green qPCR reagent, passive reference dye included

L6544

LuminoCt® SYBR® Green qPCR ReadyMix™, For fast SYBR Green quantitative PCR

KCQS00

KiCqStart® SYBR® Green qPCR ReadyMix预混液™, For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

KCQS01

KiCqStart® SYBR® Green qPCR ReadyMix预混液™, Low ROX™, for ABI and Stratagene instruments

KCQS02

KiCqStart® SYBR® Green qPCR ReadyMix预混液™, with ROX™ for ABI instruments

KCQS03

KiCqStart® SYBR® Green qPCR ReadyMix预混液™, iQ™, with fluorescein for Bio-Rad systems

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Aifeng Liu et al.
Journal of nanoscience and nanotechnology, 19(1), 91-97 (2018-10-18)
Osteoarthritis (OA) is an unavoidable degenerative disease of the human body. A relatively efficient and desirable treatment exists that leads to the ecological restoration of cartilage through the adjustments of the micro-environment of the human body and relies on its
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, Third Edition (2000)
H Wouter Wisselink et al.
Applied and environmental microbiology, 73(15), 4881-4891 (2007-06-05)
For cost-effective and efficient ethanol production from lignocellulosic fractions of plant biomass, the conversion of not only major constituents, such as glucose and xylose, but also less predominant sugars, such as l-arabinose, is required. Wild-type strains of Saccharomyces cerevisiae, the
Validation of Quantitative PCR Assays
Lovatt, A., et al.
BioPharm., March, 22-23 (2002)
T B Morrison et al.
BioTechniques, 24(6), 954-958 (1998-06-19)
Continuous fluorescence observation of amplifying DNA allows rapid and accurate quantification of initial transcript copy number. A simple and generic method for monitoring product synthesis with the double-stranded DNA dye, SYBR Green I provides initial template copy number estimation limited
查看所有相關論文

文章

Using Probe-Based Quantitative PCR (Qpcr) to Measure Gene-Level Expression

Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

條款

SYBR® Green I Dye Quantitative PCR Protocol

A protocol that can be used as a basic template for qPCR incorporating SYBR Green I DNA binding dye that is amenable to modification and applicable for use as validation for a set of primers.

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務