推薦產品
品質等級
形狀
liquid
用途
sufficient for 100 reactions
sufficient for 2000 reactions
sufficient for 500 reactions
特點
dNTPs included
hotstart
技術
qPCR: suitable
顏色
colorless
輸入
purified DNA
應用
agriculture
相容性
Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ 5
for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000
檢測方法
SYBR® Green
運輸包裝
wet ice
儲存溫度
−20°C
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相關類別
一般說明
- Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms
- ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays
- Kit is not compatible with qPCR instruments that utilize glass capillary tubes
應用
特點和優勢
- Ideal for performing high-throughput, quantitative PCR applications
- The master mix allows consistency from one reaction to the next
- Reduced preparation time and reduced risk of contamination from multiple pipetting steps
- The hot start mechanism using the JumpStart Taq antibody prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
- LuminoCt® ReadyMixes require the use of small amplicons (<200bp) for optimal results
- This master mix is compatible with commercial primer sets, including TaqMan® assays
- Assay results in as little as 25 minutes
- Unsurpassed accuracy, precision, and sensitivity
- Virtually eliminates the need to optimize assay parameters
包裝
100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL
其他說明
- At a minimum, use software to design primers for assays and ensure that qPCR amplicons are <200 bp
- Sigma recommends designing primers and probes with Beacon Designer software
- To order primers and probes from Sigma, visit: www.sigma.com/oligos
法律資訊
僅套裝組件
- 2 X
相關產品
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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文章
A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
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Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
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