跳轉至內容
Merck
全部照片(2)

重要文件

S9430

Sigma-Aldrich

SYBR®Green I核酸凝胶染液

greener alternative

10,000 × in DMSO

同義詞:

DNA stain, SYBR® green gel dye, safer gel stain

登入查看組織和合約定價
全部照片(2)

About This Item

CAS號碼:
163795-75-3
MDL號碼:
MFCD00284715
分類程式碼代碼:
12171500
NACRES:
NA.52

形狀

solution

品質等級

200

用途

1.0 mL sufficient for 100 mini-gels

環保替代產品特色

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

濃度

10,000 × in DMSO

技術

PCR: suitable

環保替代類別

Aligned,

儲存溫度

−20°C

尋找類似的產品？ 前往 產品比較指南

相關類別

一般說明

SYBR® Green I是一种特制的不对称花菁染料，用于检测核酸。这种成分由N-烷基化苯并噻唑或苯并噁唑环系统组成，通过单次甲基桥连接吡啶鎓或喹啉鎓环系统。SYBR Green I与dsDNA的小沟结合，激发波长480 nm。其荧光发射峰波长为520nm。

應用

SYBR® Green I核酸凝胶染色剂已用于：
  • dsDNA定量
  • 聚合酶链式反应（PCR），用于DNA染色
  • Comet测定技术
  • 评估精子膜的完整性
  • 扩增DNA 目视检查（环介导等温扩增（LAMP）法）
  • 用作流式细胞术中的荧光染料
  • 实时定量逆转录聚合酶链式反应（qRT-PCR），用于逆转录cDNA染色

特點和優勢

  • 用于在电泳后对琼脂糖或聚丙烯酰胺凝胶中的双链DNA染色的超灵敏染色剂
  • 它还可以检测变性琼脂糖/甲醛和聚丙烯酰胺/尿素凝胶中的ssDNA和RNA，无需任何预洗涤步骤
  • 在 Ames 试验中，它的致突变性比溴化乙锭小
  • 它对寡核苷酸的检测灵敏度是溴化乙锭的 50-100 倍
  • 适用于 DNA 数量有限的许多应用
  • SYBR® Green I 与 DNA 的结合不会抑制许多常见限制性内切酶的活性，包括 Hind III 和 EcoR I
  • 这种染色剂无需通过凝胶酶解和连接技术去除
  • SYBR Green I 是溴化乙锭染色的绿色替代产品

儲存和穩定性

将产品储存在–20 °C。稀释后的染色溶液避光可在2–8 °C保存数周或室温保存3-4天。

法律資訊

SYBR is a registered trademark of Life Technologies

相關產品

產品號碼
描述
訂價

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

201.2 °F - closed cup

閃點（°C）

94 °C - closed cup

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
SLCM2152
MKCQ6695
SLCL6245
MKCQ2272
MKCQ2547

未看到正確版本？

如果您需要一個特定的版本，您可以透過批號來尋找特定憑證。

搜尋 COA

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications
Zipper H, et al.
Nucleic Acids Research, 32(12), e103-e103 (2004)
P A Morin et al.
BioTechniques, 19(2), 223-228 (1995-08-01)
A method for resolving and visualizing genotypes for simple sequence repeat loci on short (20 cm) polyacrylamide gels is described. This method makes use of a modified vertical electrophoresis cell to allow rapid electrophoresis without variation in migration rates due
Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering
Tresoldi C, et al.
Materials Science and Engineering, C, 105, 110035-110035 (2019)
The in vitro effect of nonylphenol, propranolol, and diethylstilbestrol on quality parameters and oxidative stress in sterlet (Acipenser ruthenus) spermatozoa
Shaliutina O, et al.
Toxicology in vitro, 43, 9-15 (2017)
Loop mediated isothermal amplification of 5.8S rDNA for specific
detection of Tritrichomonas foetus
Jorge Oyhenart
Veterinary Parasitology, 193 (2013)
查看所有相關論文

條款

Hot Start dNTP protocol to reduce non-specific amplification

Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.

SeqPlex Enhanced DNA Amplification Kit (SEQXE) Protocol

The SeqPlex DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

相關內容

Complete Whole Transcriptome Amplification Kit Protocol (WTA2)

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

Complete Whole Transcriptome Amplification Kit Protocol (WTA2)

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務