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P9187

Sigma-Aldrich

OPD

SIGMAFAST™, peroxidase substrate, chromogenic, tablet

同義詞:

OPD过氧化物酶底物

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83

product name

SIGMAFAST™ OPD, tablet

形狀

tablet

品質等級

200

溶解度

water: 0.5 tablet/10 mL, clear, colorless

儲存溫度

2-8°C

相關類別

應用

SIGMAFAST™ OPD (o-苯二胺二盐酸盐)片剂用作酶免疫测定(EIA)检测氧化物酶活性的可溶底物。OPD作为EIA底物是因为它有高灵敏度。 使用OPD的EIA可以在计时分析中读数或用稀释的酸性溶液终止反应，过后在读数。
SIGMAFAST OPD已用于唾液病毒样颗粒(VLP)结合测定。它还已用于开发检测大鼠血清样品中的利妥昔单抗的酶联免疫吸附测定(ELISA)板。

特點和優勢

SIGMAFAST™ OPD片剂不需要另外的缓冲液或步骤来制备活性底物溶液。

注意

该底物吸湿且光敏感

重構

一片OPD片剂和一片尿素过氧化氢/缓冲液片剂，溶解在20ml水中，提供20ml即用底物。 如果按照该方法溶解片剂，得到的终浓度是0.4 mg/ml OPD, 0.4 mg/ml尿素过氧化氢和0.05 M磷酸柠檬酸盐，pH5.0。片剂是独立铝膜包装。
将每组片剂溶解在20mL去离子水中，就能生成即用型含有OPD和尿素过氧化氢缓冲溶液。

法律資訊

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Skin Sens. 1 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

分析證明 (COA)

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The noninvasive early detection of biomarkers for Alzheimer's disease (AD) is essential for the development of specific treatment strategies. This paper proposes an advanced method for fabricating highly ordered and flexible nanopillar-based electrochemical biosensors by the combination of soft/photolithography and
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Ecology and evolution, 7(11), 3645-3656 (2017-06-16)
Oceanic islands share several environmental characteristics that have been shown to drive convergent evolutionary changes in island organisms. One change that is often assumed but has seldom been examined is the evolution of weaker immune systems in island species. The
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ELISA Procedures

ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.

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