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Key Documents

P8787

Sigma-Aldrich

邻苯二胺 二盐酸盐

chromogenic, tablet

同義詞:

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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About This Item

線性公式:
C6H4(NH2)2·2HCl
CAS號碼:
分子量::
181.06
Beilstein:
3912045
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83

product name

邻苯二胺 二盐酸盐, tablet, 4 mg substrate per tablet

品質等級

形狀

tablet

mp

258 °C (dec.) (lit.)

儲存溫度

2-8°C

SMILES 字串

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI 密鑰

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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一般說明

邻苯二胺二盐酸盐(OPD)是一种显色底物,因为利用辣根过氧化物酶缀合物,因此可用于酶联免疫吸附试验(ELISA)程序。底物产生可溶的最终产物,其颜色为橙棕色,并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应,并在492 nm处读取。

應用

在酶联免疫吸附试验(ELISA)中,邻苯二胺盐酸盐用作辣根过氧化物酶偶联二抗的底物。

重構

将 1 片片剂溶于 pH 值为 5.0的0.05M磷酸盐 - 柠檬酸盐缓冲液中至所需浓度(通常使用0.4mg / ml的OPD浓度)。临用前,向 100 mL 底物缓冲液中加入 40μl 新鲜过氧化氢(浓度为 30%)。片剂单独包装在铝箔包装中。

訊號詞

Warning

危險分類

Aquatic Chronic 2 - Carc. 2 - Muta. 2 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3


分析證明 (COA)

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Kaustuv Nayak et al.
Virology, 558, 13-21 (2021-03-12)
India is one of the most affected countries by COVID-19 pandemic; but little is understood regarding immune responses to SARS-CoV-2 in this region. Herein we examined SARS-CoV-2 neutralizing antibodies, IgG, IgM, IgA and memory B cells in COVID-19 recovered individual
Esther Steiner et al.
BMC veterinary research, 5, 45-45 (2009-12-24)
Porcine circovirus type 2 (PCV2) is a dominant causative agent of postweaning multisystemic wasting syndrome (PMWS), a multifactorial disease complex with putative immunosuppressive characteristics. Little is known about adaptive PCV2-specific immune responses in infected pigs. Therefore, the T and B
Siddhartha K Bhaumik et al.
Viruses, 11(1) (2019-01-02)
The re-emergence of Zika virus (ZIKV) in the western hemisphere has most significantly affected dengue virus (DENV) endemic regions. Due to the geographical overlap between these two closely related flaviviruses, numerous individuals who suffered ZIKV infection during recent outbreaks may
Cassandra Koole et al.
The Journal of pharmacology and experimental therapeutics, 353(1), 52-63 (2015-01-30)
The glucagon-like peptide-1 receptor (GLP-1R) is a class B G protein-coupled receptor that has a critical role in the regulation of glucose homeostasis, principally through the regulation of insulin secretion. The receptor system is highly complex, able to be activated
Florian Krammer et al.
Clinical and vaccine immunology : CVI, 21(8), 1153-1163 (2014-06-20)
Emerging H7N9 influenza virus infections in Asia have once more spurred the development of effective prepandemic H7 vaccines. However, many vaccines based on avian influenza viruses--including H7--are poorly immunogenic, as measured by traditional correlates of protection. Here we reevaluated sera

文章

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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