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一般說明
磷酸柠檬酸缓冲液是一种常用的缓冲液，与可溶性辣根过氧化物酶底物一起使用，如邻苯二胺二盐酸（OPD）或四甲基联苯胺（TMB）。它的pH值为5.0，具有出色的缓冲能力。柠檬酸磷酸盐缓冲片可配制0.05 M、pH 5.0的柠檬酸磷酸盐缓冲液。这些片剂提供了一种快速、方便、准确的合成柠檬酸磷酸酯缓冲溶液的方法。它消除了在缓冲液制备中称重单个成分的耗时和繁琐的过程。磷酸盐-柠檬酸缓冲片由最高质量的成分制造，以严格的物理和化学规格，以确保性能和批次间的一致性。pH 5.0的磷酸盐-柠檬酸盐缓冲液片剂已在免疫测定中配制为底物缓冲液。
應用
- Characterization of nettle leaves (Urtica dioica) as a novel source of protease for clotting dromedary milk by non-destructive methods.：在酶促提取过程中使用磷酸-柠檬酸缓冲液保持植物来源酶的稳定性和活性，说明它在乳制品天然混凝剂开发中的重要作用(Bouazizi et al., 2022)。
- A rapid, low pH, nutrient stress, assay to determine the bactericidal activity of compounds against non-replicating Mycobacterium tuberculosis.：使用磷酸-柠檬酸缓冲液，研究开发一种低pH营养应激检测方法，评估抗菌剂对结核休眠菌的杀菌疗效，提供重要药物开发工具(Early et al., 2019)。
- Measurement of Free Iodine in Different Formulations of Povidone-Iodine Eye Drops 5.：用磷酸-柠檬酸缓冲液检测滴眼液中碘的稳定性和释放，说明它确保护眼产品疗效和安全性的重要作用(Prado et al., 2019)。
重構
在25℃下，一片溶于100 mL去离子水中可得到0.05 M磷酸盐-柠檬酸盐缓冲液，pH 5.0。室温储存。
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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