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P9029

Sigma-Aldrich

邻苯二胺

peroxidase substrate, chromogenic, ≥98.0%, powder

同義詞:

1,2-二氨基苯, 1,2-苯二胺, OPD

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About This Item

經驗公式（希爾表示法）:
C6H8N2
CAS號碼:
95-54-5
分子量：:
108.14
Beilstein:
606074
EC號碼:
202-430-6
MDL號碼:
MFCD00007721
分類程式碼代碼:
12352204
PubChem物質ID:
24899004
NACRES:
NA.83

product name

邻苯二胺, Peroxidase substrate, ≥98.0%, powder

蒸汽密度

3.7 (vs air)

蒸汽壓力

0.01 mmHg ( 25 °C)

化驗

≥98.0%

形狀

powder

顏色

white to off-white

bp

256-258 °C

mp

98-102 °C

溶解度

ethanol: 50 mg/mL, clear, colorless to faintly yellow

螢光

λex 337 nm; λem 417 nm (Pyruvic acid derivative)

儲存溫度

2-8°C

SMILES 字串

Nc1ccccc1N

InChI

1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2

InChI 密鑰

GEYOCULIXLDCMW-UHFFFAOYSA-N

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相關類別

一般說明

o-苯二胺(OPD)自由基是过氧物酶底物，适用于酶联免疫吸附测定(ELISA)程序。底物产生可溶的产物，其颜色为橙棕色，并且可以在 450nm 下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应，并在492 nm处读取。

應用

o-苯二胺已用于微量滴定分析的透明质酸量化。也用于酶联免疫吸附测定(ELISA)显色。

注意

可能会在存储期间变黑。

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類（WGK）

WGK 3

閃點（°F）

276.8 °F - closed cup

閃點（°C）

136 °C - closed cup

分析證明 (COA)

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Kelsey E Noll et al.
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Host genetic factors play a fundamental role in regulating humoral immunity to viral infection, including influenza A virus (IAV). Here, we utilize the Collaborative Cross (CC), a mouse genetic reference population, to study genetic regulation of variation in antibody response
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C.Rodriguez, et al.
The Veterinary Journal, 207(, pages=), 160-163 (2016)
Expression of hyaluronan synthase 2 or hyaluronidase 1 differentially affect the growth rate of transplantable colon carcinoma cell tumors
Jacobson A, et al.
International Journal of Cancer. Journal International Du Cancer, 102(3), 212-219 (2002)
Patrick A Julien et al.
Beilstein journal of organic chemistry, 13, 2160-2168 (2017-11-09)
We provide the first in situ and real-time study of the effect of milling frequency on the course of a mechanochemical organic reaction conducted using a vibratory shaker (mixer) ball mill. The use of in situ Raman spectroscopy for real-time
Development of a chicken-derived antivenom against the taipan snake (Oxyuranus scutellatus) venom and comparison with an equine antivenom
Navarro D, et al.
Toxicon, 120(3), 1-8 (2016)
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文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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