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Merck
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文件

P7288

Sigma-Aldrich

邻苯二胺 二盐酸盐

chromogenic, tablet

同義詞:

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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About This Item

線性公式:
C6H4(NH2)2·2HCl
CAS號碼:
615-28-1
分子量：:
181.06
Beilstein:
3912045
EC號碼:
210-418-7
MDL號碼:
MFCD00012966
分類程式碼代碼:
12352204
PubChem物質ID:
24898837
NACRES:
NA.83

product name

邻苯二胺 二盐酸盐, tablet, 20 mg substrate per tablet

形狀

tablet

mp

258 °C (dec.) (lit.)

儲存溫度

2-8°C

SMILES 字串

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI 密鑰

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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相關類別

一般說明

邻苯二胺二盐酸盐（OPD）是一种显色底物，因为利用辣根过氧化物酶缀合物，因此可用于酶联免疫吸附试验（ELISA）程序。底物产生可溶的最终产物，其颜色为橙棕色，并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应，并在492 nm处读取。

應用

o-Phenylenediamine dihydrochloride has been used:
  • as a substrate for the eosinophil peroxidase (EPO) assay in bronchoalveolar lavage fluid (BALF)
  • in cell adhesion molecule-enzyme immunoassay (CAM-EIA) to analyze the amounts of E-selectin induced by test compounds (LPS or solutions and materials) on endothelial cells
  • in ultra-sensitive enzyme-linked immunosorbent assay (ELISA) to measure prolactin and luteinizing hormone (LH)

重構

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

訊號詞

Warning

危險分類

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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Ronald E Unger et al.
Biomaterials, 35(10), 3180-3187 (2014-01-25)
Endotoxin, also known as lipopolysaccharide (LPS) produced by bacteria can be present in any liquid or on any biomaterial even if the material is sterile. Endotoxin in mammals can cause fever, inflammation, cell and tissue damage and irreversible septic shock
D Sano et al.
Journal of applied microbiology, 109(6), 1923-1928 (2010-08-26)
Transmission routes of noroviruses, leading aetiological agents of acute gastroenteritis, are rarely verified when outbreaks occur. Because the destination of norovirus particles being firmly captured by micro-organisms could be totally different from that of those particles moving freely, micro-organisms with
C A Ison
Methods in molecular medicine, 66, 317-329 (2001-01-01)
Neisseria meningitidis is an obligate human pathogen. When it interacts with the host, it can establish a commensal relationship or can, on a minority of occasions, invade and cause systemic disease. Protection against systemic disease, particularly for serogroup A and
Cassandra Koole et al.
The Journal of pharmacology and experimental therapeutics, 353(1), 52-63 (2015-01-30)
The glucagon-like peptide-1 receptor (GLP-1R) is a class B G protein-coupled receptor that has a critical role in the regulation of glucose homeostasis, principally through the regulation of insulin secretion. The receptor system is highly complex, able to be activated
Florian Krammer et al.
Clinical and vaccine immunology : CVI, 21(8), 1153-1163 (2014-06-20)
Emerging H7N9 influenza virus infections in Asia have once more spurred the development of effective prepandemic H7 vaccines. However, many vaccines based on avian influenza viruses--including H7--are poorly immunogenic, as measured by traditional correlates of protection. Here we reevaluated sera
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文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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